Western Blot (WB)
(Western blot analysis of TCF7L1 expression in COLO320 whole cell lysates (lane 1). TCF7L1 at 63KD was detected using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
anti-Human TCF7L1 Polyclonal Antibody | anti-TCF7L1 antibody
Anti-TCF7L1 Antibody
Concentration :0.1-0.5 ug/ml
Tested Species: Hu
Immunohistochemistry (Paraffin-embedded Section) :
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
By Heat: Boiling the paraffin sections in 10 mm citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. .
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Western blot analysis of TCF7L1 expression in COLO320 whole cell lysates (lane 1). TCF7L1 at 63KD was detected using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of TCF7L1 expression in COLO320 whole cell lysates (lane 1). TCF7L1 at 63KD was detected using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(TCF7L1 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(TCF7L1 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Background: Transcription factor 7-like 1 (T-cell specific, HMG-box), also known as TCF7L1, is a human gene. This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence. [provided by RefSeq, Nov 2010]
Uniprot Description
TCF3: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. Down-regulates NQO1, leading to increased mitomycin c resistance. Belongs to the TCF/LEF family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: nucleoplasm; nucleus; transcription factor complex
Molecular Function: beta-catenin binding; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin
Research Articles on TCF7L1
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Product Notes
The TCF7L1 tcf7l1 (Catalog #AAA177921) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCF7L1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF7L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration :0.1-0.5 ug/ml Tested Species: Hu Immunohistochemistry (Paraffin-embedded Section) : Concentration: 0.5-1ug/ml Tested Species: Hu Antigen Retrieval: By Heat Tested Species: In house tested species with positive results. By Heat: Boiling the paraffin sections in 10 mm citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the TCF7L1 tcf7l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF7L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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