Rabbit anti-Human CDK3 Polyclonal Antibody | anti-CDK3 antibody

CDK3, NT (Y19) (CDK3, CDKN3, Cyclin-dependent kinase 3, Cell division protein kinase 3) (HRP)

Cat. Num. AAA6284384

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CDK3; Polyclonal Antibody; NT (Y19) (CDK3; CDKN3; Cyclin-dependent kinase 3; Cell division protein kinase 3) (HRP); Cell division protein kinase 3; anti-CDK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDK3
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDK3 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CDK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human CDK3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of CDK3(arrow) using rabbit polyclonal CDK3 Antibody (N-term Y19). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK3 Antibody (N-term Y19), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using CDK3 Antibody (N-term Y19)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CDK3 antibody

Antibodies; Protein Kinases

References

Bullrich, F., et al., Cancer Res. 55(6):1199-1205 (1995). Meyerson, M., et al., EMBO J. 11(8):2909-2917 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4557439
• NCBI GeneID: 1018
• NCBI Accession #: NP_001249.1
• NCBI GenBank Nucleotide #: NM_001258.2
• UniProt Accession #: Q00526
• Molecular Weight: 35,046 Da
• NCBI Official Full Name: cyclin-dependent kinase 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3; cell division protein kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3
• UniProt Entry Name: CDK3_HUMAN

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.

Protein type: Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 17q25.1

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: mitosis; cell proliferation; G0 to G1 transition; regulation of cell cycle; cell division; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle