Rabbit anti-Mouse TBCC Polyclonal Antibody | anti-TBCC antibody

TBCC, NT (TBCC, Tubulin-specific chaperone C, Tubulin-folding cofactor C) (Biotin)

Cat. Num. AAA6354009

• Gene Names: TBCC; CFC
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBCC; Polyclonal Antibody; NT (TBCC; Tubulin-specific chaperone C; Tubulin-folding cofactor C) (Biotin); Tubulin-folding cofactor C; anti-TBCC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TBCC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human TBCC.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TBCC Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the TBCC antibody detected the TBCC protein (arrow).)

Related Product Information for anti-TBCC antibody

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Product Categories/Family for anti-TBCC antibody

Antibodies; Tubulin

References

Hage-Sleiman, R., et al. BMC Cancer 10, 135 (2010): Nature 447(7145):661-678(2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Bartolini, F., et al. J. Biol. Chem. 277(17):14629-14634(2002). Tian, G., et al. Cell 86(2):287-296(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4507373
• NCBI GeneID: 6903
• NCBI Accession #: NP_003183.1
• NCBI GenBank Nucleotide #: NM_003192.2
• UniProt Accession #: Q15814; Q53Y43; Q5T787
• Molecular Weight: 39,248 Da
• NCBI Official Full Name: tubulin-specific chaperone C
• NCBI Official Synonym Full Names: tubulin folding cofactor C
• NCBI Official Symbol: TBCC
• NCBI Official Synonym Symbols: CFC
• NCBI Protein Information: tubulin-specific chaperone C; tubulin-folding cofactor C
• UniProt Protein Name: Tubulin-specific chaperone C
• Protein Family: TBCC domain-containing protein
• UniProt Gene Name: TBCC
• UniProt Synonym Gene Names: CFC
• UniProt Entry Name: TBCC_HUMAN

NCBI Description

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008]

Uniprot Description

TBCC: Tubulin-folding protein; involved in the final step of the tubulin folding pathway. Belongs to the TBCC family.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: microtubule; cytoskeleton; cytoplasm; photoreceptor connecting cilium

Molecular Function: GTPase activity; chaperone binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell morphogenesis; post-chaperonin tubulin folding pathway