Mouse Importin subunit alpha-3, KPNA3 ELISA Kit | KPNA3 elisa kit

Mouse Importin subunit alpha-3, KPNA3 ELISA Kit

Cat. Num. AAA2602730

• Gene Names: Kpna3; IPOA4
• Reactivity: Mouse
• Synonyms: Importin subunit alpha-3; KPNA3; Mouse Importin subunit alpha-3; KPNA3 ELISA Kit; KPNA3 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant KPNA3 concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KPNA3 elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse KPNA3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 6680596
• NCBI GeneID: 16648
• NCBI Accession #: NP_032492.1
• NCBI GenBank Nucleotide #: NM_008466.5
• UniProt Accession #: O35344
• Molecular Weight: 57,773 Da
• NCBI Official Full Name: importin subunit alpha-4
• NCBI Official Synonym Full Names: karyopherin (importin) alpha 3
• NCBI Official Symbol: Kpna3
• NCBI Official Synonym Symbols: IPOA4
• NCBI Protein Information: importin subunit alpha-4; qip2; importin alpha Q2; importin subunit alpha-3; karyopherin subunit alpha-3
• UniProt Protein Name: Importin subunit alpha-4
• UniProt Gene Name: Kpna3
• UniProt Synonym Gene Names: Qip2; Qip2
• UniProt Entry Name: IMA4_MOUSE

Uniprot Description

KPNA3: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. Belongs to the importin alpha family.

Protein type: Chaperone; Nuclear import; Karyopherin; Adaptor/scaffold

Cellular Component: cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein transporter activity

Biological Process: protein transport; transport; protein import into nucleus