Rabbit Tau Polyclonal Antibody | anti-tau antibody

Tau, phosphorylated (Ser400) (Tau Protein, Microtubule-associated Protein, MAP)

Cat. Num. AAA628612

• Gene Names: tau; BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Tau; Polyclonal Antibody; phosphorylated (Ser400) (Tau Protein; Microtubule-associated Protein; MAP); Anti -Tau; anti-tau antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human Tau protein at ~85kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.

• Applicable Applications for anti-tau antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.05-0.2ug/ml; Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding serine 400 of human Tau, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of recombinant human Tau-441 fusion protein using: Lane 1: MBS628612 pre-incubated with non-phosphopeptide Lane 2: MBS628612 pre-incubated with phosphopeptide Lane 3: MBS628612 Lane 4: Purified rabbit IgG (whole molecule) control. Secondary antibody: IgG, H&L (HRP) Pab Gt xRb (I1904-46Q). The signal was developed with LumiSensorTMHRP Substrate Kit.)

Western Blot (WB)

(Western Blot analysis of mouse brain tissue lysate using: Lane 1: Purified rabbit IgG (whole molecule control Lane 2: MBS628612 Lane 3: MBS628612 pre-incubated with phosphopeptide. Secondary antibody: IgG, H&L (HRP) Pab Gt xRb (I1904-46Q). The signal was developed with LumiSensorTMHRP Substrate Kit.)

Western Blot (WB)

(Western Blot analysis of rat brain lysate using MBS628612 at 1ug/ml. The signal was developed with IRDyeTM 800-conjugated goat anti-rabbit IgG.)

Related Product Information for anti-tau antibody

Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which ranging from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal portion. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3b transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer's disease (AD). The PHFs are also found in the lesions of other central nervous system disorders.

Product Categories/Family for anti-tau antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21357661
• NCBI GeneID: 326116
• NCBI Accession #: NP_651575.1
• NCBI GenBank Nucleotide #: NM_143318.2
• NCBI Official Full Name: tau
• NCBI Official Symbol: tau
• NCBI Official Synonym Symbols: BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
• NCBI Protein Information: CG31057 gene product from transcript CG31057-RA; tau; CG31057-PA; tau-PA
• Protein Family: Tautomerase