Rabbit TANK Polyclonal Antibody | anti-TANK antibody

TANK Antibody

Cat. Num. AAA840581

• Gene Names: TANK; ITRAF; TRAF2; I-TRAF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: TANK; Polyclonal Antibody; TANK Antibody; TANK (NT); TRAF family member-associated NF-kappaB activator; I-TRAF; anti-TANK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) affinity purified rabbit anti-TANK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-TANK antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Western blotting (0.5-4 ug/ml) and in immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. Mouse small intestine tissue lysate can be used as a positive control.
• Immunogen: Synthetic peptide surrounding amino acid 413 of human TANK
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-TANK antibody

Background: Members of TRAF family associate with activated TNF-R and CD40 and have been implicated to induce nuclear translocation of NFkB. TANK can bind to all three TRAFs and synergizing with TRAF2 to activate the NFkB signal cascade.

NCBI and Uniprot Product Information

• NCBI GI #: 312433953
• NCBI GeneID: 10010
• NCBI Accession #: NP_001186064.1
• NCBI GenBank Nucleotide #: NM_001199135.1
• UniProt Accession #: Q92844; Q7Z4J6; Q92885; D3DPB5
• Molecular Weight: 13,626 Da
• NCBI Official Full Name: TRAF family member-associated NF-kappa-B activator isoform a
• NCBI Official Synonym Full Names: TRAF family member-associated NFKB activator
• NCBI Official Symbol: TANK
• NCBI Official Synonym Symbols: ITRAF; TRAF2; I-TRAF
• NCBI Protein Information: TRAF family member-associated NF-kappa-B activator
• UniProt Protein Name: TRAF family member-associated NF-kappa-B activator
• Protein Family: Tankyrase
• UniProt Gene Name: TANK
• UniProt Synonym Gene Names: ITRAF; TRAF2; I-TRAF
• UniProt Entry Name: TANK_HUMAN

NCBI Description

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

TANK: Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF- kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity. Interacts with TBK1 (via TRAF-C domain). Interacts with TRAF1 (via TRAF-C domain). Interacts with TRAF2 (via TRAF-C domain); the interaction is disrupted by the phosphorylation of TANK by IKBKE. Interacts with TRAF3 (via TRAF-C domain); the interaction with TRAF3 is weaker than the interactions with TRAF1 and TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; metal ion binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 4 signaling pathway