Human netrin 4 ELISA Kit | NTN4 elisa kit

Human Netrin-4, Ntn4 ELISA Kit

Cat. Num. AAA700010

• Gene Names: NTN4; PRO3091
• Reactivity: Human
• Synonyms: netrin 4; Human Netrin-4; Ntn4 ELISA Kit; FLJ23180; PRO3091; Netrin-4; beta-netrin; NTN4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NTN4. No significant cross-reactivity or interference between Human NTN4 and analogues was observed.
• Sequence Length: 628

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NTN4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for netrin-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any netrin-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for netrin-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of netrin-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 93204871
• NCBI GeneID: 59277
• NCBI Accession #: NP_067052.2
• NCBI GenBank Nucleotide #: NM_021229.3
• UniProt Accession #: Q9HB63; Q658K9; Q7L3F1; Q7L9D6; Q7Z5B6; Q9BZP1; Q9NT44; Q9P133; B2RNC2
• Molecular Weight: 70,071 Da
• NCBI Official Full Name: netrin-4
• NCBI Official Synonym Full Names: netrin 4
• NCBI Official Symbol: NTN4
• NCBI Official Synonym Symbols: PRO3091
• NCBI Protein Information: netrin-4; beta-netrin; hepar-derived netrin-like protein
• UniProt Protein Name: Netrin-4
• Protein Family: Netrin
• UniProt Gene Name: NTN4
• UniProt Entry Name: NET4_HUMAN

NCBI Description

NTN4 belongs to a family of proteins related to laminins (see LAMA1, MIM 150320) Koch et al. (2000) [PubMed 11038171].[supplied by OMIM, Mar 2008]

Uniprot Description

Function: May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.1 PublicationManual assertion based on experiment in:Ref.1