Rabbit anti-Mouse; Rat TAK1 Polyclonal Antibody | anti-TAK1 antibody

TAK1 Antibody: RPE

Cat. Num. AAA8000695

• Gene Names: MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
• Reactivity: Mouse; Rat
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: TAK1; Polyclonal Antibody; TAK1 Antibody: RPE; Rabbit Anti-Human TAK1 Polyclonal; MAP3K7 Antibody; TAK1 Antibody; Mitogen-activated protein kinase kinase kinase 7 Antibody; TGF-beta-activated kinase 1 Antibody; Transforming growth factor-beta-activated kinase 1 Antibody; MEKK7 Antibody; anti-TAK1 antibody

• Host: Rabbit
• Reactivity: Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects 67.2 kDa, 40 kDa band is a possible degradation product.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 579

• Applicable Applications for anti-TAK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: TAK1
• Immunogen: Synthetic peptide of Human TAK1 (400-500 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Cell Membrane; Peripheral Membrane Protein; Cytoplasmic Side
• Conjugate: RPE
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat liver lysate showing detection of ~67.2 kDa TAK1 protein using Rabbit Anti-TAK1 Polyclonal Antibody (SPC-736). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TAK1 Polyclonal Antibody (SPC-736) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.2 kDa. Other Band(s): ~40 kDa potential degradation product.)

Related Product Information for anti-TAK1 antibody

TAK1 encoded by the gene MAP3K7, is a protein-serine/threonine kinase that is activated by proinflammatory cytokines and in response to physical/chemical stress, including UVR, osmotic- and oxidative stress. It is a mediator of TRAF6 and TGF-beta signal transduction, and activates IKBKB and MAPK8 in response to TRAF6 signalling. It also stimulates NFkappaB activation and activation of the p38 MAPK pathway. It is responsible for controlling a variety of cell functions such as transcription and apoptosis. TAK1 is important for TGF-beta1 regulation of MMP9 and the metastatic potential of certain breast cancer cell lines.

Product Categories/Family for anti-TAK1 antibody

Antibodies; Cell Signaling; Protein Phosphorylation; Serine/Threonine Kinases; MAPK Pathway; Cell Signaling; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; NFkB Pathway; Cell Signaling; Protein Phosphorylation; Tyrosine Kinases; Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4507361
• NCBI GeneID: 6885
• NCBI Accession #: NP_003179.1
• NCBI GenBank Nucleotide #: NM_003188.3
• UniProt Accession #: O43318; O43317; O43319; Q5TDN2; Q5TDN3; Q5TDT7; Q9NTR3; Q9NZ70; B2RE27; E1P523
• Molecular Weight: 53,740 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: CSCF; FMD2; TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.