Mouse Lactate ELISA Kit
Mouse Lactate ELISA Kit
Reactivity
Mouse
Synonyms
Lactate; Mouse Lactate ELISA Kit; Lactate elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of LAT. No significant cross-reactivity or interference between LAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LAT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 mmol/L
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lactate elisa kit
Intended Uses: This LAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LAT. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: LAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti- LAT antibody and an LAT-HRP conjugate. The assay sample and buffer are incubated together with LAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LAT concentration since LAT from samples and LAT-HRP conjugate compete for the anti- LAT antibody binding site. Since the number of sites is limited, as more sites are occupied by LAT from the sample, fewer sites are left to bind LATHRP conjugate. A standard curve is p lotted relating the intensity of the color (O.D.) to the concentration of standards. The LAT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti- LAT antibody and an LAT-HRP conjugate. The assay sample and buffer are incubated together with LAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LAT concentration since LAT from samples and LAT-HRP conjugate compete for the anti- LAT antibody binding site. Since the number of sites is limited, as more sites are occupied by LAT from the sample, fewer sites are left to bind LATHRP conjugate. A standard curve is p lotted relating the intensity of the color (O.D.) to the concentration of standards. The LAT concentration in each sample is interpolated from this standard curve.
