Rabbit anti-Human TAF9 Polyclonal Antibody | anti-TAF9 antibody

TAF9, NT (TAF9, TAF2G, TAFII31, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G, STAF31/32, Transcription initiation factor TFIID 31kD subunit, Transcription initiation factor TFIID 32kD subunit) (MaxLigh

Cat. Num. AAA6353455

• Gene Names: TAF9; TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF9; Polyclonal Antibody; NT (TAF9; TAF2G; TAFII31; Transcription initiation factor TFIID subunit 9; RNA polymerase II TBP-associated factor subunit G; STAF31/32; Transcription initiation factor TFIID 31kD subunit; Transcription initiation factor TFIID 32kD subunit) (MaxLigh; Transcription initiation factor TFIID 32kD subunit; anti-TAF9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TAF9 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TAF9.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-TAF9 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-TAF9 antibody

Antibodies; Transcription Factors

References

Kim, D.H., et al. BMB Rep 42(7):411-417(2009). Sengupta, T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(11):4213-4218(2009). Liu, X., et al. Mol. Cell. Biol. 28(1):108-121(2008). McKeegan, K.S., et al. Mol. Cell. Biol. 27(19):6782-6793(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 62865614
• NCBI GeneID: 6880
• NCBI Accession #: NP_001015892.1; NP_003178.1
• NCBI GenBank Nucleotide #: NM_001015892.1
• UniProt Accession #: Q16594; Q5U0D1; Q9BTS1; D3DWA3
• Molecular Weight: 28,974 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 9
• NCBI Official Synonym Full Names: TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
• NCBI Official Symbol: TAF9
• NCBI Official Synonym Symbols: TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32
• NCBI Protein Information: transcription initiation factor TFIID subunit 9; RNA polymerase II TBP-associated factor subunit G; transcription initiation factor TFIID 31 kD subunit; transcription initiation factor TFIID 31 kDa subunit; transcription initiation factor TFIID 32 kDa sub
• UniProt Protein Name: Transcription initiation factor TFIID subunit 9
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF9
• UniProt Synonym Gene Names: TAF2G; TAFII31; TAFII-31; TAFII31; TAFII-32; TAFII32
• UniProt Entry Name: TAF9_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

TAFII31: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.

Protein type: EC 2.7.4.3; Transcription initiation complex; DNA-binding; Acetyltransferase; Kinase, other

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; transcription activator binding; protein heterodimerization activity; p53 binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; protein stabilization; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to DNA damage stimulus; negative regulation of apoptosis