Rabbit anti-Human TAF9 Polyclonal Antibody | anti-TAF9 antibody

TAF9 Antibody

Cat. Num. AAA7045687

• Gene Names: TAF9; TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: TAF9; Polyclonal Antibody; TAF9 Antibody; Transcription initiation factor TFIID subunit 9; RNA polymerase II TBP-associated factor subunit G; STAF31/32; Transcription initiation factor TFIID 31 kDa subunit; TAFII-31; TAFII31; Transcription initiation factor TFIID 32 kDa subunit; TAFII-32; TAFII32; TAF2G; anti-TAF9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 264

• Applicable Applications for anti-TAF9 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC: 1:20-1:200
• Species: Human
• Immunogen: Recombinant human Transcription initiation factor TFIID subunit 9 protein (1-172AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:MCF-7 whole cell lysate,293T whole cell lysate,Hela whole cell lysate,K562 whole cell lysate,A431 whole cell lysateAll lanes: AK6 antibody at 0.59ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 21,20kDaObserved band size: 20 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7045687 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin tissue using MBS7045687 at dilution 1:100)

Related Product Information for anti-TAF9 antibody

Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 62865614
• NCBI GeneID: 6880
• NCBI Accession #: NP_001015892.1
• NCBI GenBank Nucleotide #: NM_001015892.1
• UniProt Accession #: Q16594; Q5U0D1; Q9BTS1; D3DWA3
• Molecular Weight: 28,974 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 9
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 9
• NCBI Official Symbol: TAF9
• NCBI Official Synonym Symbols: TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32
• NCBI Protein Information: transcription initiation factor TFIID subunit 9
• UniProt Protein Name: Transcription initiation factor TFIID subunit 9
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF9
• UniProt Synonym Gene Names: TAF2G; TAFII31; TAFII-31; TAFII31; TAFII-32; TAFII32
• UniProt Entry Name: TAF9_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

TAFII31: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex

Molecular Function: ATPase binding; DNA binding; histone acetyltransferase activity; p53 binding; protein binding; transcription activator binding; transcription coactivator activity

Biological Process: box C/D snoRNP assembly; negative regulation of apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell growth; positive regulation of transcription from RNA polymerase II promoter; protein stabilization; response to DNA damage stimulus; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter