Rabbit TAF6 Polyclonal Antibody | anti-TAF6 antibody
TAF6 Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TAF6; Polyclonal Antibody; TAF6 Antibody; TAF2E; E 70/85kD; TAF 6; TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD; TAF6 RNA polymerase II; TATA box binding protein associated factor; ; 80kDa; TAFII 70; TAFII80; TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD; TATA box binding protein (TBP) associated factor; RNA polymerase II; TBP associated factor 6; Transcription initiation factor TFIID 70 kDa subunit; Transcription initiation factor TFIID subunit 6; anti-TAF6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TAF6 Antibody detects endogenous levels of total TAF6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TAF6 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the TAF6 family.
Post Translational Modifications
In cells undergoing apoptosis, cleaved in a caspase-dependent manner to produce a 40 kDa product.
Subunit Structure
TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP, TAF1/TAFII250, TAF9/TAFII31 AND TAF12/TAFII20. The TAF6/TAFII70-TAF9/TAFII31 heterodimer forms an octamer complex with the TAF4B/TFII105-TAF12/TFIID20 heterodimer. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Also interacts with the GTFs, TFIIEalpha/GTF2E1 and TFIIFalpha/GTF2F1. Component of the TFTC-HAT complex. Isoform 1: Interacts with TP53/p53. Isoform 4: Part of a TFIID-like complex which lacks TAF9. Isoform 4: Not part of the TFTC. Isoform 4: Interacts with TP53/p53.
Function
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.Transcriptional regulator which acts primarily as a positive regulator of transcription. Recruited to the promoters of a number of genes including GADD45A and CDKN1A/p21, leading to transcriptional up-regulation and subsequent induction of apoptosis. Also up-regulates expression of other genes including GCNA/ACRC, HES1 and IFFO1. In contrast, down-regulates transcription of MDM2. Acts as a transcriptional coactivator to enhance transcription of TP53/p53-responsive genes such as DUSP1. Can also activate transcription and apoptosis independently of TP53. Drives apoptosis via the intrinsic apoptotic pathway by up-regulating apoptosis effectors such as BCL2L11/BIM and PMAIP1/NOXA.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Mol.Wt.: 73kDa(Calculated)
UniProt Protein Name
Transcription initiation factor TFIID subunit 6
Protein Family
UniProt Gene Name
TAF6
UniProt Synonym Gene Names
TAF2E; TAFII70
UniProt Entry Name
TAF6_HUMAN
Uniprot Description
TAF6: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF6 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription initiation complex
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; viral reproduction; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression; negative regulation of cell cycle
Similar Products
Product Notes
The TAF6 taf6 (Catalog #AAA9629329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAF6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TAF6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the TAF6 taf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
