Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS628489, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS628489 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human BRD4 Polyclonal Antibody | anti-BRD4 antibody
BRD4, NT (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1) (FITC)
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
BRD4; Polyclonal Antibody; NT (Bromodomain-containing Protein 4; Protein HUNK1; HUNK1) (FITC); anti-BRD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BRD4.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-BRD4 antibody
Immunohistochemistry (IHC) Paraffin, FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal regin of human BRD4 (O60885). Cellular Localization: Nucleus and chromosome.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS628489, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS628489 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS628489, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS628489 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-BRD4 antibody
References
1. Gagnon, D. et al. J Virol. May; 83(9): 4127-4139(2009). 2. Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002). 3. French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001). 4. Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000). 5. David Gagnon, et al. J. Virol., Feb 2009; 10.1128/JVI.02468-08. (Applications: WB)(E-pub).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
152kDa
NCBI Official Full Name
bromodomain-containing protein 4 isoform short
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
NCBI Protein Information
bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
UniProt Protein Name
Bromodomain-containing protein 4
Protein Family
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN
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Product Notes
The BRD4 brd4 (Catalog #AAA6466032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRD4, NT (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRD4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BRD4 brd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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