Rabbit anti-Human TAF4B Polyclonal Antibody | anti-TAF4B antibody

Rabbit Polyclonal to Human TAF4B

Cat. Num. AAA248778

• Gene Names: TAF4B; SPGF13; TAF2C2; TAFII105
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: TAF4B; Polyclonal Antibody; Rabbit Polyclonal to Human TAF4B; Anti-TAF4B Antibody (aa654-683) IHC-plus; TAF(II)105; TAFII105; TAF2C2; TAFII-105; Human TAF4B; anti-TAF4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human TAF4B
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 867

• Applicable Applications for anti-TAF4B antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from C-terminal region of human TAF4B.
• Immunogen Type: Synthetic peptide
• Immunogen: TAF4B antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human TAF4B.
• Antigen Modification: aa654-683
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-TAF4B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Flow Cytometry (FC/FACS)

(TAF4B Antibody (C-term) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(TAF4B Antibody (C-term) Western blot of K562 cell line lysates (35 ug/lane). This demonstrates the TAF4B antibody detected the TAF4B protein (arrow).)

NCBI and Uniprot Product Information

• NCBI GI #: 656985069
• NCBI GeneID: 6875
• NCBI Accession #: NP_001280654.1
• NCBI GenBank Nucleotide #: NM_001293725.1
• UniProt Accession #: Q92750; Q29YA4; Q29YA5
• Molecular Weight: 63,364 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 4B isoform 1
• NCBI Official Synonym Full Names: TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa
• NCBI Official Symbol: TAF4B
• NCBI Official Synonym Symbols: SPGF13; TAF2C2; TAFII105
• NCBI Protein Information: transcription initiation factor TFIID subunit 4B; TAF(II)105; TATA box binding protein (TBP)-associated factor 4B; transcription initiation factor TFIID 105 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C2, 105kD
• UniProt Protein Name: Transcription initiation factor TFIID subunit 4B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF4B
• UniProt Synonym Gene Names: TAF2C2; TAFII105; TAF(II)105; TAFII-105; TAFII105
• UniProt Entry Name: TAF4B_HUMAN

NCBI Description

TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

TAF4B: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Belongs to the TAF4 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm; nucleolus

Molecular Function: NF-kappaB binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; oogenesis; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; spermatogenesis; gene expression

Disease: Spermatogenic Failure 13