Rabbit anti-Human TAF1C Polyclonal Antibody | anti-TAF1C antibody

TAF1C Antibody

Cat. Num. AAA1494754

• Gene Names: TAF1C; SL1; TAFI95; TAFI110; MGC:39976
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: TAF1C; Polyclonal Antibody; TAF1C Antibody; RNA polymerase I-specific TBP-associated factor 110 kDa; TAFI110; TATA box-binding protein-associated factor 1C; TBP-associated factor 1C; Transcription initiation factor SL1/TIF-IB subunit C; anti-TAF1C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 843

• Applicable Applications for anti-TAF1C antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human TATA box-binding protein-associated factor RNA polymerase I subunit C protein (70-320aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6566 / sc-292391
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1494754 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland using MBS1494754 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of MCF-7 cells using MBS1494754 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-TAF1C antibody

Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.

NCBI and Uniprot Product Information

• NCBI GI #: 341926281
• NCBI GeneID: 9013
• NCBI Accession #: NP_001230085.1
• NCBI GenBank Nucleotide #: NM_001243156.1
• UniProt Accession #: Q15572; Q59F67; Q8N6V3; B7Z5K5; B7Z5S4; B7Z908; B7Z9L7
• Molecular Weight: 92,284 Da
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit C isoform 3
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor, RNA polymerase I, C
• NCBI Official Symbol: TAF1C
• NCBI Official Synonym Symbols: SL1; TAFI95; TAFI110; MGC:39976
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit C
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit C
• Protein Family: TATA box-binding protein-associated factor
• UniProt Gene Name: TAF1C
• UniProt Synonym Gene Names: TAFI110; TBP-associated factor 1C
• UniProt Entry Name: TAF1C_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]

Uniprot Description

TAF1C: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; RNA polymerase I transcription factor complex

Molecular Function: protein binding

Biological Process: chromatin silencing at rDNA; gene expression; negative regulation of gene expression, epigenetic; positive regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter