Testing Data
(Fig.1. Time-lapse SIM images of live U2OS cells stained with MitoTracker Green (green, 400 nM) or TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) (magenta, 15 muM). The result shows that the photobleaching resistance of TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) is better than that of Mitotracker green.)
Pro Live-Cell Mitochondrion Staining Kit
Pro Live-Cell Mitochondrion Staining Kit (Deep Red Fluorescence with Super Resolution)
Synonyms
Pro Live-Cell Mitochondrion; Pro Live-Cell Mitochondrion Staining Kit (Deep Red Fluorescence with Super Resolution); Pro Live-Cell Mitochondrion staining kit
Features & Benefits
Optimized staining protocol for labeling Mitochondrion in mammalian living and fixed cells.
Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
Proprietary MitoRed (Ex/Em = 647/661nm)-high specificity, low background and excellent photostability.
Low levels of cytotoxicity.
Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
Proprietary MitoRed (Ex/Em = 647/661nm)-high specificity, low background and excellent photostability.
Low levels of cytotoxicity.
Usage Notes
* Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.
* To avoid MitoRed adsorbed on glass that may affect the signal to noise ratio. Nunc Glass Bottom Dishes is recommended for better image quality.
* To avoid MitoRed adsorbed on glass that may affect the signal to noise ratio. Nunc Glass Bottom Dishes is recommended for better image quality.
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
Testing Data
(Fig.1. Time-lapse SIM images of live U2OS cells stained with MitoTracker Green (green, 400 nM) or TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) (magenta, 15 muM). The result shows that the photobleaching resistance of TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) is better than that of Mitotracker green.)
Testing Data
(Fig.1. Time-lapse SIM images of live U2OS cells stained with MitoTracker Green (green, 400 nM) or TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) (magenta, 15 muM). The result shows that the photobleaching resistance of TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) is better than that of Mitotracker green.)
Testing Data
(Fig.2. Confocal images of U2OS cells stained with TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) (15 uM) before and after cell fixation. The result shows cell fixation had little effect on the)
Testing Data
(Fig.2. Confocal images of U2OS cells stained with TraKine Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) (15 uM) before and after cell fixation. The result shows cell fixation had little effect on the)
Related Product Information for Pro Live-Cell Mitochondrion staining kit
Description: This Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Mitochondrion in mammalian living and fixed cells with high specificity and low background. The proprietary probe (Ex/Em: 647/661nm) can selectively recognize and label Mitochondrion and cell fixation had little effect on the mitochondrial labeling pattern of the proprietary probe. It is especially suitable for long-term super-resolution imaging.
Background: Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Lysosomes and mitochondria are crucial dynamic organelles that participate extensively in many important cellular processes. Their dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases.
Background: Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Lysosomes and mitochondria are crucial dynamic organelles that participate extensively in many important cellular processes. Their dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases.
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Product Notes
The Pro Live-Cell Mitochondrion (Catalog #AAA9718805) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pro Live-Cell Mitochondrion, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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