Rabbit anti-Human TAF10 Polyclonal Antibody | anti-TAF10 antibody

TAF10, ID (TAF10, TAF2A, TAF2H, TAFII30, Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30kD subunit) (MaxLight 750)

Cat. Num. AAA6353334

• Gene Names: TAF10; TAF2A; TAF2H; TAFII30
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF10; Polyclonal Antibody; ID (TAF10; TAF2A; TAF2H; TAFII30; Transcription initiation factor TFIID subunit 10; STAF28; Transcription initiation factor TFIID 30kD subunit) (MaxLight 750); Transcription initiation factor TFIID 30kD subunit; anti-TAF10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TAF10 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 120-150 amino acids selected from the Central region of human TAF10.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-TAF10 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.

Product Categories/Family for anti-TAF10 antibody

Antibodies; Transcription Factors

References

Egloff, S., et al. J. Biol. Chem. 285(27):20564-20569(2010). Ma, Y., et al. J. Biol. Chem. 285(13):9813-9822(2010). Zhao, Y., et al. Mol. Cell 29(1):92-101(2008). Hao, H., et al. Oncogene 26(57):7872-7884(2007). Couture, J.F., et al. Nat. Struct. Mol. Biol. 13(2):140-146(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5454106
• NCBI GeneID: 6881
• NCBI Accession #: NP_006275.1
• NCBI GenBank Nucleotide #: NM_006284.3
• UniProt Accession #: Q12962; O00703; Q13175; Q6FH13
• Molecular Weight: 21,711 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 10
• NCBI Official Synonym Full Names: TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
• NCBI Official Symbol: TAF10
• NCBI Official Synonym Symbols: TAF2A; TAF2H; TAFII30
• NCBI Protein Information: transcription initiation factor TFIID subunit 10; STAF28; TAF(II)30; TAFII-30; TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD; transcription initiation factor TFIID 30 kD subunit; transcription initiation factor TFIID 30 kDa
• UniProt Protein Name: Transcription initiation factor TFIID subunit 10
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF10
• UniProt Synonym Gene Names: TAF2A; TAF2H; TAFII30; TAF(II)30; TAFII-30; TAFII30
• UniProt Entry Name: TAF10_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF10: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF10 family.

Protein type: Nuclear receptor co-regulator; Transcription initiation complex

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; enzyme binding; transcription coactivator activity; estrogen receptor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; regulation of transcription, DNA-dependent; transcription initiation; RNA elongation from RNA polymerase II promoter; gene expression; protein homooligomerization; histone deubiquitination