Rabbit anti-Human, Mouse TAF10 Polyclonal Antibody | anti-TAF10 antibody

TAF10 Antibody

Cat. Num. AAA9407652

• Gene Names: TAF10; TAF2A; TAF2H; TAFII30
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: TAF10; Polyclonal Antibody; TAF10 Antibody; TAF2A; TAF2H; TAFII30; anti-TAF10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total TAF10 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 2.1 mg/ml (varies by lot)
• Sequence Length: 218

• Applicable Applications for anti-TAF10 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:25-1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
• Target Name: TAF10
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 12%SDS-PAGE Lysate: 40ug Human lung cancer tissuePrimary antibody: 1/400 dilution Secondary antibody dilution: 1/8000Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/35.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/35.)

Related Product Information for anti-TAF10 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Product Categories/Family for anti-TAF10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5454106
• NCBI GeneID: 6881
• NCBI Accession #: NP_006275.1
• NCBI GenBank Nucleotide #: NM_006284.3
• UniProt Accession #: Q12962; O00703; Q13175; Q6FH13
• Molecular Weight: 21,711 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 10
• NCBI Official Synonym Full Names: TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
• NCBI Official Symbol: TAF10
• NCBI Official Synonym Symbols: TAF2A; TAF2H; TAFII30
• NCBI Protein Information: transcription initiation factor TFIID subunit 10
• UniProt Protein Name: Transcription initiation factor TFIID subunit 10
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF10
• UniProt Synonym Gene Names: TAF2A; TAF2H; TAFII30; TAF(II)30; TAFII-30; TAFII30
• UniProt Entry Name: TAF10_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF10: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF10 family.

Protein type: Nuclear receptor co-regulator; Transcription initiation complex

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: nucleoplasm; transcription factor TFIID complex; PCAF complex; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone acetyltransferase activity; transcription coactivator activity; estrogen receptor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression; protein homooligomerization; histone deubiquitination