Rabbit anti-Human, mouse TAF15 Polyclonal Antibody | anti-TAF15 antibody

TAF15 Antibody

Cat. Num. AAA9215048

• Gene Names: TAF15; Npl3; RBP56; TAF2N; TAFII68
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TAF15; Polyclonal Antibody; TAF15 Antibody; TATA-binding protein-associated factor 2N; 68 kDa TATA-binding protein-associated factor; TAF(II)68; TAFII68; RNA-binding protein 56; RBP56; TAF2N; anti-TAF15 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Positions: 371-400
• Sequence Length: 589

• Applicable Applications for anti-TAF15 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from Hela,293T cell line (from left to right),using TAF15 Antibody(MBS9215048). MBS9215048 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using TAF15 antibody.)

Related Product Information for anti-TAF15 antibody

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).

References

Morohoshi F.,et al.Genomics 38:51-57(1996).
Bertolotti A.,et al.EMBO J. 15:5022-5031(1996).
Morohoshi F.,et al.Gene 221:191-198(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4507353
• NCBI GeneID: 8148
• NCBI Accession #: NP_003478.1
• NCBI GenBank Nucleotide #: NM_003487.3
• UniProt Accession #: Q92804; Q86X94; Q92751; B2R837
• Molecular Weight: 61830
• NCBI Official Full Name: TATA-binding protein-associated factor 2N isoform 2
• NCBI Official Synonym Full Names: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• NCBI Official Symbol: TAF15
• NCBI Official Synonym Symbols: Npl3; RBP56; TAF2N; TAFII68
• NCBI Protein Information: TATA-binding protein-associated factor 2N
• UniProt Protein Name: TATA-binding protein-associated factor 2N
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF15
• UniProt Synonym Gene Names: RBP56; TAF2N; TAF(II)68; TAFII68
• UniProt Entry Name: RBP56_HUMAN

NCBI Description

This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

TAF15: RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Oncoprotein

Chromosomal Location of Human Ortholog: 17q11.1-q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; nucleotide binding

Biological Process: positive regulation of transcription, DNA-dependent

Disease: Chondrosarcoma, Extraskeletal Myxoid