Rabbit TACC3 Polyclonal Antibody | anti-TACC3 antibody

TACC3 Antibody

Cat. Num. AAA9613513

• Gene Names: TACC3; ERIC1; ERIC-1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (92%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: TACC3; Polyclonal Antibody; TACC3 Antibody; ERIC 1; ERIC-1; ERIC1; MGC117382; MGC133242; OTTHUMP00000113796; TACC3_HUMAN; Transforming acidic coiled coil containing protein 3; Transforming acidic coiled-coil-containing protein 3; anti-TACC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (92%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TACC3 Antibody detects endogenous levels of total TACC3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TACC3 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TACC3, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Subunit Structure: Interacts with microtubules. Interacts with CKAP5 independently of clathrin. Interacts with CKAP5 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; TACC3 (phosphorylated at Ser-558 by AURKA) and CLTC are proposed to form a composite microtubule interaction surface. Interacts with CCDC100/CEP120. The coiled coil C-terminal region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.
• Similarity: Belongs to the TACC family.
• Subcellular Location: Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.; Note: In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of TACC3 in lysates of HeLa  , using TACC3 Antibody(AF4806).)

Related Product Information for anti-TACC3 antibody

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 5454102
• NCBI GeneID: 10460
• NCBI Accession #: NP_006333.1
• NCBI GenBank Nucleotide #: NM_006342.2
• UniProt Accession #: Q9Y6A5; Q3KQS5; Q9UMQ1
• Molecular Weight: 90,360 Da
• NCBI Official Full Name: transforming acidic coiled-coil-containing protein 3
• NCBI Official Synonym Full Names: transforming, acidic coiled-coil containing protein 3
• NCBI Official Symbol: TACC3
• NCBI Official Synonym Symbols: ERIC1; ERIC-1
• NCBI Protein Information: transforming acidic coiled-coil-containing protein 3
• UniProt Protein Name: Transforming acidic coiled-coil-containing protein 3
• Protein Family: Transforming acidic coiled-coil-containing protein
• UniProt Gene Name: TACC3
• UniProt Synonym Gene Names: ERIC1
• UniProt Entry Name: TACC3_HUMAN

NCBI Description

This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells. [provided by RefSeq, Nov 2011]

Uniprot Description

TACC3: Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. May be involved in the control of cell growth and differentiation. May contribute to cancer. Interacts with microtubules. Interacts with CCDC100/CEP120. The coiled coil C-terminus region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2. Interacts with GCN5L2 and PCAF. Up-regulated in various cancer cell lines. Belongs to the TACC family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: centrosome; cytoplasm

Molecular Function: protein domain specific binding; protein binding

Biological Process: cell proliferation; regulation of microtubule-based process; cytoplasmic sequestering of transcription factor; astral microtubule organization and biogenesis; neurogenesis; regulation of cell cycle; response to hypoxia; hemopoiesis; microtubule cytoskeleton organization and biogenesis; cerebral cortex development; interkinetic nuclear migration

Disease: Bladder Cancer