Human IL-5 ELISA Kit | IL5 elisa kit

Human IL-5 ELISA Kit

Cat. Num. AAA8244594

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-5; Human IL-5 ELISA Kit; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; IL5 elisa kit

• Reactivity: Human
• Specificity: The Human IL-5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-5 proteins within the range of 3.9 pg/ml - 250 pg/ml.
• Sequence Length: 134

• Applicable Applications for IL5 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
• Sensitivity: 2 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for IL5 elisa kit

Principle of the Assay||The Human IL-5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

NCBI and Uniprot Product Information

• NCBI GI #: 124341
• NCBI GeneID: 3567
• UniProt Accession #: P05113; Q13840
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: Interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding

Biological Process: activation of MAPKK activity; axon guidance; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway