Western Blot (WB)
(Western blot analysis of T150A Antibody (Center) in Jurkat cell line lysates (35ug/lane). T150A (arrow) was detected using the purified Pab.)
Rabbit T150A Polyclonal Antibody | anti-TMEM150A antibody
T150A Antibody (Center)
Gene Names
TMEM150A; TM6P1; TMEM150
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
T150A; Polyclonal Antibody; T150A Antibody (Center); Transmembrane protein 150A; Transmembrane protein 150; TMEM150A; TMEM150; anti-TMEM150A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This T150A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the Central region of human T150A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
81-108
Sequence Length
218
Applicable Applications for anti-TMEM150A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of T150A Antibody (Center) in Jurkat cell line lysates (35ug/lane). T150A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of T150A Antibody (Center) in Jurkat cell line lysates (35ug/lane). T150A (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with T150A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with T150A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(T150A Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(T150A Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TMEM150A antibody
T150A is possible role in fasting-induced catabolism.
Product Categories/Family for anti-TMEM150A antibody
References
Zhang,J. Biochim. Biophys. Acta 1492 (1), 280-284 (2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28835
NCBI Official Full Name
transmembrane protein 150A
NCBI Official Synonym Full Names
transmembrane protein 150A
NCBI Official Symbol
TMEM150A
NCBI Official Synonym Symbols
TM6P1; TMEM150
NCBI Protein Information
transmembrane protein 150A
UniProt Protein Name
Transmembrane protein 150A
Protein Family
UniProt Gene Name
TMEM150A
UniProt Synonym Gene Names
TMEM150
UniProt Entry Name
T150A_HUMAN
Uniprot Description
TMEM150A: Possible role in fasting-induced catabolism. Belongs to the DRAM/TMEM150 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: lysosome; integral to membrane; plasma membrane
Biological Process: apoptotic nuclear changes; catabolic process
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Product Notes
The TMEM150A tmem150a (Catalog #AAA9214899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 81-108. The T150A Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's T150A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the TMEM150A tmem150a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T150A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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