Rabbit anti-Human T Plastin/PLS3 Polyclonal Antibody | anti-PLS3 antibody

Anti-T Plastin/PLS3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107508

• Gene Names: PLS3; BMND18; T-plastin
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: T Plastin/PLS3; Polyclonal Antibody; Anti-T Plastin/PLS3 Antibody; Rabbit Polyclonal; T Plastin/PLS3 Antibody; Rabbit PAb; Antigen Affinity Purified; plastin 3; Anti-BMND18 Antibody; Anti-T-plastin Antibody; anti-PLS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human T Plastin/PLS3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PLS3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:200-1:1000; IP: 1-5uL/mg of lysate
• Immunogen: Recombinant Human T Plastin/PLS3 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human T Plastin/PLS3 (rh T Plastin/PLS3). T Plastin/PLS3 specific IgG was purified by Human T Plastin/PLS3 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PLS3 rabbit polyclonal antibody at 1:500 dilution Lane A: Raji Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: HT1080 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PLS3 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(PLS3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PLS3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PLS3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa Observed band size :60 kDa)

Related Product Information for anti-PLS3 antibody

PLS3, also known as plastin 3, belongs to the plastin family. Members of this family are actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. There are two ubiquitous plastin isoforms in humans: L and T. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). PLS3 contains 2 actin-binding domains, 4 CH (calponin-homology) domains and 2 EF-hand domains. It is expressed in a variety of organs, including muscle, brain, uterus and esophagus.

References

Lin CS. et al., 1993, J Biol Chem 268 (4): 2781-92.Goldstein D. et al., 1985, Cancer Res. 45 (2): 5643-7.Arpin M. et al., 1995, J Cell Biol. 127 (2): 1995-2008.

NCBI and Uniprot Product Information

• NCBI GI #: 226694201
• NCBI GeneID: 5358
• UniProt Accession #: P13797; Q86YI6; A8K579; B1AQ09; B4DGB4; B7Z6M1
• Molecular Weight: 630
• NCBI Official Full Name: Plastin-3
• NCBI Official Synonym Full Names: plastin 3
• NCBI Official Symbol: PLS3
• NCBI Official Synonym Symbols: BMND18; T-plastin
• NCBI Protein Information: plastin-3; T fimbrin; T plastin
• UniProt Protein Name: Plastin-3
• Protein Family: Plastin
• UniProt Gene Name: PLS3
• UniProt Entry Name: PLST_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; actin binding

Disease: Bone Mineral Density Quantitative Trait Locus 18