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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse T-Box Protein 21 Polyclonal Antibody | anti-TBX21 antibody

Polyclonal Antibody to T-Box Protein 21 (TBX21)

Gene Names
Tbx21; TBT1; Tbet; Tblym
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
T-Box Protein 21; Polyclonal Antibody; Polyclonal Antibody to T-Box Protein 21 (TBX21); anti-TBX21 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TBX21. It has beenselected for its ability to recognize TBX21 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/ml (varies by lot)
Applicable Applications for anti-TBX21 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
TBX21 (Lys160~Arg399)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant TBX21 (Lys160~Arg399) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084537
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,852 Da
NCBI Official Full Name
T-box transcription factor TBX21
NCBI Official Synonym Full Names
T-box 21
NCBI Official Symbol
Tbx21
NCBI Official Synonym Symbols
TBT1; Tbet; Tblym
NCBI Protein Information
T-box transcription factor TBX21
UniProt Protein Name
T-box transcription factor TBX21
UniProt Gene Name
Tbx21
UniProt Synonym Gene Names
Tbet; Tblym; T-box protein 21

Uniprot Description

TBX21: Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. T-cell specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11|11 D

Cellular Component: cell soma; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; transcription regulatory region DNA binding

Biological Process: cellular response to organic substance; lymphocyte migration; negative regulation of interleukin-2 production; negative regulation of T-helper 17 cell differentiation; negative regulation of T-helper 17 cell lineage commitment; negative regulation of T-helper 2 cell cytokine production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-templated; proteasomal ubiquitin-dependent protein catabolic process; regulation of immune response; regulation of T cell differentiation; regulation of transcription, DNA-templated; T cell differentiation; transcription, DNA-dependent

Research Articles on TBX21

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Product Notes

The TBX21 tbx21 (Catalog #AAA2028673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to T-Box Protein 21 (TBX21) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's T-Box Protein 21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the TBX21 tbx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T-Box Protein 21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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