Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Angiogenin Polyclonal Antibody | anti-ANG antibody
Polyclonal Antibody to Angiogenin (ANG)
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene. [provided by RefSeq, Jun 2009]
Uniprot Description
ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.
Protein type: Cell cycle regulation; EC 3.1.27.-; Extracellular matrix; Motility/polarity/chemotaxis; Nucleolus; RNA-binding; Ribonuclease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14 C1|14 26.37 cM
Cellular Component: angiogenin-PRI complex; basal lamina; cell soma; cytoplasmic vesicle; extracellular region; extracellular space; growth cone; nucleus
Molecular Function: actin binding; copper ion binding; DNA binding; endonuclease activity; heparin binding; hydrolase activity; nuclease activity; nucleic acid binding; peptide binding; protein homodimerization activity; receptor binding; ribonuclease activity; RNA binding
Biological Process: activation of phospholipase A2 activity; activation of phospholipase C activity; activation of protein kinase B activity; angiogenesis; cell differentiation; cell migration; central nervous system development; diacylglycerol biosynthetic process; multicellular organism development; negative regulation of smooth muscle cell proliferation; negative regulation of translation; positive regulation of endothelial cell proliferation; positive regulation of phosphorylation; positive regulation of protein secretion; response to hormone; response to hypoxia; rRNA transcription
Research Articles on ANG
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Product Notes
The ANG ang (Catalog #AAA2027789) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Angiogenin (ANG) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiogenin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ANG ang for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiogenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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