Rabbit T-bet/Tbx21 Polyclonal Antibody | anti-TBX21 antibody

Anti-T-bet/Tbx21 antibody

Cat. Num. AAA176063

• Gene Names: TBX21; TBET; T-PET; T-bet; TBLYM
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: T-bet/Tbx21; Polyclonal Antibody; Anti-T-bet/Tbx21 antibody; T-box transcription factor TBX21; T-box 21; T bet antibody; T box 21 antibody; T box expressed in T cells antibody; T box protein 21 antibody; T box transcription factor TBX21 antibody; T cell specific T box transcription factor antibody; T cell specific T box transcription factor T bet antibody; T PET antibody; T-box protein 21 antibody; T-box transcription factor TBX21 antibody; T-cell-specific T-box transcription factor T-bet antibody; TBET antibody; TBLYM antibody; TBX 21 antibody; TBX21 antibody; TBX21_HUMAN antibody; TPET antibody; Transcription factor TBLYM antibody; anti-TBX21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 535

• Applicable Applications for anti-TBX21 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet (522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-T-bet/Tbx21 antibody, MBS176063, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human TBX21,34.7KD(162aa tag+ V381-N535)Lane 1: Recombinant Human TBX21 Protein 5ngLane 2: Recombinant Human TBX21 Protein 2.5ngLane 3: Recombinant Human TBX21 Protein 1.25ng )

Related Product Information for anti-TBX21 antibody

Description: Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human, Mouse, Rat.
Background: TBX21(T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang(2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al.(2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer(NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al.(2002) observed reduced expression of the T(H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 12230772
• NCBI GeneID: 30009
• UniProt Accession #: Q9UL17
• Molecular Weight: 58,328 Da
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: TBX21
• NCBI Official Synonym Symbols: TBET; T-PET; T-bet; TBLYM
• NCBI Protein Information: T-box transcription factor TBX21; T-box protein 21; T-box expressed in T cells; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet
• UniProt Protein Name: T-box transcription factor TBX21
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX21
• UniProt Synonym Gene Names: TBET; TBLYM; T-box protein 21
• UniProt Entry Name: TBX21_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX21: Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. T-cell specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell soma; nucleus

Molecular Function: transcription factor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to virus; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation

Disease: Asthma, Nasal Polyps, And Aspirin Intolerance