Western Blot (WB)
(Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human SYTL2 Polyclonal Antibody | anti-SYTL2 antibody
SYTL2 Antibody (Center)
IHC-P~~1:50~100
FC~~1:10~50
Western Blot (WB)
(Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(SYTL2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYTL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SYTL2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYTL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Kesari, A., et al. Am. J. Pathol. 173(5):1476-1487(2008)
Chavas, L.M., et al. Structure 16(10):1468-1477(2008)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]
Uniprot Description
SYTL2: Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol- 4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon- containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca(2+) while binding to PIP2 is Ca(2+) insensitive. 12 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Lipid-binding; Motility/polarity/chemotaxis; GAPs, Rab; GAPs
Chromosomal Location of Human Ortholog: 11q14
Cellular Component: nucleoplasm; extrinsic to plasma membrane; Golgi apparatus; membrane; cytoplasm; plasma membrane; melanosome; nucleus
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; phosphatidylserine binding; neurexin binding; Rab GTPase binding; phosphatase binding
Biological Process: vesicle-mediated transport; intracellular protein transport; exocytosis; vesicle docking during exocytosis
Research Articles on SYTL2
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Product Notes
The SYTL2 sytl2 (Catalog #AAA9210686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 840-868. The SYTL2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SYTL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the SYTL2 sytl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYTL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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