Western Blot (WB)
(Western Blot analysis of Hela cell line lysates (35ug/lane )using MBS646694. SYTL2 (arrow) was detected using MBS646694.)
Rabbit anti-Human SYTL2 Polyclonal Antibody | anti-SYTL2 antibody
SYTL2, ID (SYTL2, KIAA1597, SGA72M, SLP2, SLP2A, Synaptotagmin-like protein 2, Breast cancer-associated antigen SGA-72M, Exophilin-4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis of Hela cell line lysates (35ug/lane )using MBS646694. SYTL2 (arrow) was detected using MBS646694.)
Western Blot (WB)
(Western Blot analysis of Hela cell line lysates (35ug/lane )using MBS646694. SYTL2 (arrow) was detected using MBS646694.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded brain tissue using MBS646694 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646694 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded brain tissue using MBS646694 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646694 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using MBS646694 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using MBS646694 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]
Uniprot Description
Function: Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon-containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca2+ while binding to PIP2 is Ca2+ insensitive. Ref.1 Ref.9 Ref.10
Subunit structure: Monomer. Binds NRXN1. Interacts with RAB27B
By similarity. Binds RAB27A that has been activated by GTP-binding. Ref.1 Ref.11
Subcellular location: Isoform 1: Cytoplasm. Cell membrane. Note: Recruited on vesicular structures in cytotoxic T-lymphocytes (CTL) by RAB27A. Ref.1 Ref.9 Ref.10Isoform 4: Cell membrane. Note: In the pancreatic alpha cells distributed in both peripheral and anterior regions. Localizes on the glucagon granules in the cell periphery. Ref.1 Ref.9 Ref.10
Tissue specificity: Isoform 1 is expressed in hematopoietic lineages with a strong expression in CD4 and CD8 T-lymphocytes. It is also widely expressed in nonhematopoietic tissues. Isoform 5 is expressed only in nonhematopoietic tissues. Isoform 4 is expressed in pancreatic alpha cells. Ref.1 Ref.9 Ref.10
Domain: The RabBD domain mediates interaction with RAB27A and recruitment on to vesicular structures in cytotoxic T-lymphocytes (CTL). Ref.1 Ref.9The C2 1 domain mediates binding to phosphatidylserine (PS) and phosphatidylinositol 4,5-bisphosphate (PIP2) and localization to the cell membrane. Ref.1 Ref.9
Post-translational modification: Isoform 1 is highly susceptible to proteolytic degradation and is stabilized by the interaction with RAB27A.
Sequence similarities: Contains 2 C2 domains.Contains 1 RabBD (Rab-binding) domain.
Sequence caution: The sequence AAR25619.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAA90989.1 differs from that shown. Reason: Probable cloning artifact.The sequence BAB13423.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAB13423.1 differs from that shown. Reason: Frameshift at position 13. The sequence BAB13423.1 differs from that shown. Reason: Sequencing errors.The sequence BAD18516.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on SYTL2
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Product Notes
The SYTL2 sytl2 (Catalog #AAA646694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SYTL2, ID (SYTL2, KIAA1597, SGA72M, SLP2, SLP2A, Synaptotagmin-like protein 2, Breast cancer-associated antigen SGA-72M, Exophilin-4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SYTL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the SYTL2 sytl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYTL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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