Rabbit anti-Human SYTL2 Polyclonal Antibody | anti-SYTL2 antibody

SYTL2, ID (SYTL2, KIAA1597, SGA72M, SLP2, SLP2A, Synaptotagmin-like protein 2, Breast cancer-associated antigen SGA-72M, Exophilin-4) (MaxLight 650)

Cat. Num. AAA6353091

• Gene Names: SYTL2; EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SYTL2; Polyclonal Antibody; ID (SYTL2; KIAA1597; SGA72M; SLP2; SLP2A; Synaptotagmin-like protein 2; Breast cancer-associated antigen SGA-72M; Exophilin-4) (MaxLight 650); Exophilin-4; anti-SYTL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SYTL2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-SYTL2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 840-868 in the central region of human SYTL2, Swiss/UniProt Accession: Q9HCH5.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Hela cell line lysates (35ug/lane)usingMBS646694. SYTL2 (arrow) was detected usingMBS646694.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded brain tissue using MBS646694 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646694 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS646694 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SYTL2 antibody

Antibodies; Cancer Markers

References

1. Menasche, G., et al. Blood 112(13):5052-5062(2008). 2. Kesari, A., et al. Am. J. Pathol. 173(5):1476-1487(2008). 3. Chavas, L.M., et al. Structure 16(10):1468-1477(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 244790013
• NCBI GeneID: 54843
• NCBI Accession #: NP_001156423.1
• NCBI GenBank Nucleotide #: NM_001162951.2
• UniProt Accession #: Q9HCH5; Q2YDA7; Q6TV07; Q6ZN59; Q6ZVC5; B3KRS3; B4DJT5; B4DKW3; B4DQ26; B7SA85; B7ZLX6; B7ZLX7
• Molecular Weight: 84,651 Da
• NCBI Official Full Name: synaptotagmin-like protein 2 isoform g
• NCBI Official Synonym Full Names: synaptotagmin like 2
• NCBI Official Symbol: SYTL2
• NCBI Official Synonym Symbols: EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151
• NCBI Protein Information: synaptotagmin-like protein 2
• UniProt Protein Name: Synaptotagmin-like protein 2
• Protein Family: Synaptotagmin-like protein
• UniProt Gene Name: SYTL2
• UniProt Synonym Gene Names: KIAA1597; SGA72M; SLP2; SLP2A

NCBI Description

The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]

Uniprot Description

Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon-containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca2+ while binding to PIP2 is Ca2+ insensitive.