Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Kallikrein-7 (KLK7) Standard Curve.)
Mouse Kallikrein-7 (KLK7) ELISA Kit | KLK7 elisa kit
Mouse Kallikrein-7 (KLK7) ELISA Kit
Gene Names
Klk7; SCCE; Prss6
Reactivity
Mouse
Synonyms
Kallikrein-7 (KLK7); Mouse Kallikrein-7 (KLK7) ELISA Kit; KLK7; PRSS6; SCCE; kallikrein 7 (chymotryptic; stratum corneum); protease; serine; 6; signal protein; stratum corneum chymotryptic enzyme; KLK7 elisa kit
Reactivity
Mouse
Specificity
Mouse Kallikrein-7 (KLK7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KLK7. No significant cross-reactivity or interference between Mouse KLK7 and analogues was observed.
Sequence Length
249
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Kallikrein-7 (KLK7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Kallikrein-7 (KLK7) Standard Curve.)
Related Product Information for KLK7 elisa kit
Description: This Mouse Kallikrein-7 (KLK7) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK7 in samples. An antibody specific for KLK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Kallikrein-related peptidase 7, also known as KLK7, Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Background: Kallikrein-related peptidase 7, also known as KLK7, Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kallikrein-7 preproprotein
NCBI Official Synonym Full Names
kallikrein related-peptidase 7 (chymotryptic, stratum corneum)
NCBI Official Symbol
Klk7
NCBI Official Synonym Symbols
SCCE; Prss6
NCBI Protein Information
kallikrein-7
UniProt Protein Name
Kallikrein-7
Protein Family
UniProt Gene Name
Klk7
UniProt Synonym Gene Names
Prss6; Scce
NCBI Description
This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, May 2016]
Uniprot Description
May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
Research Articles on KLK7
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Product Notes
The Mouse KLK7 klk7 (Catalog #AAA9717814) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717814 ELISA Kit recognizes Mouse KLK7. It is sometimes possible for the material contained within the vial of "Kallikrein-7 (KLK7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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