Rabbit SYT10 Polyclonal Antibody | anti-SYT10 antibody

SYT10 Antibody

Cat. Num. AAA9610269

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SYT10; Polyclonal Antibody; SYT10 Antibody; Synaptotagmin X; Synaptotagmin-10; SYT10_HUMAN; SytX; anti-SYT10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SYT10 antibody detects endogenous levels of total SYT10
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 523

• Applicable Applications for anti-SYT10 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human SYT10
• Tissue Specificity: Expressed only in pancreas, lung and kidney.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis SYT10 using COS7 whole cell lysates)

Immunofluorescene (IF)

(MBS9610269 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-SYT10 antibody

Function: Ca2+ sensor specifically required for the Ca2+-dependent exocytosis of secretory vesicles containing IGF1 in neurons of the olfactory bulb. Exocytosis of IGF1 is required for sensory perception of smell. Not involved in Ca2+-dependent synaptic vesicle exocytosis (By similarity). Acts through Ca2+ and phospholipid binding to the C2 domain: Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (By similarity).
Subunit Structure: Homodimer; disulfide-linked via the cysteine motif. Can also form heterodimers with SYT3, SYT6, SYT7 and SYT9.
Similarity: The cysteine motif mediates homo-or heterodimer formation via formation of disulfide bonds.The first C2 domain mediates Ca2+-dependent phospholipid binding. Belongs to the synaptotagmin family.

NCBI and Uniprot Product Information

• NCBI GI #: 39752671
• NCBI GeneID: 341359
• NCBI Accession #: NP_945343.1
• NCBI GenBank Nucleotide #: NM_198992.3
• UniProt Accession #: Q6XYQ8; Q495U2
• Molecular Weight: Observed: 59 kDa; Predicted: 60 kDa
• NCBI Official Full Name: synaptotagmin-10
• NCBI Official Synonym Full Names: synaptotagmin 10
• NCBI Official Symbol: SYT10
• NCBI Protein Information: synaptotagmin-10
• UniProt Protein Name: Synaptotagmin-10
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT10
• UniProt Synonym Gene Names: SytX

Uniprot Description

Ca2+ sensor specifically required for the Ca2+-dependent exocytosis of secretory vesicles containing IGF1 in neurons of the olfactory bulb. Exocytosis of IGF1 is required for sensory perception of smell. Not involved in Ca2+-dependent synaptic vesicle exocytosis (). Acts through Ca2+ and phospholipid binding to the C2 domain: Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis ().