Western Blot (WB)
(Western blot analysis of extracts from Rat brain lysate, using RAB11FIP2 antibody.)
Rabbit anti-Human, Mouse RAB11FIP2 Polyclonal Antibody | anti-RAB11FIP2 antibody
RAB11FIP2 Antibody
Gene Names
RAB11FIP2; nRip11; Rab11-FIP2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAB11FIP2; Polyclonal Antibody; RAB11FIP2 Antibody; KIAA0941; NRip11; RAB11 family interacting protein 2 (class I); Rab11 family-interacting protein 2; RAB11 FIP2 long isoform; Rab11-FIP2; RFIP2; RFIP2_HUMAN; anti-RAB11FIP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB11FIP2 antibody detects endogenous levels of total RAB11FIP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
532
Applicable Applications for anti-RAB11FIP2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane. Recycling Endosome Membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Rat brain lysate, using RAB11FIP2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Rat brain lysate, using RAB11FIP2 antibody.)
Immunofluorescene (IF)
(MBS9605824 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605824 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells using RAB11FIP2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells using RAB11FIP2 antibody.)
Related Product Information for anti-RAB11FIP2 antibody
Function: A Rab11 effector binding preferentially phosphatidylinositol 3, 4, 5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
Subunit Structure: Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3.
Post-translational Modifications: Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
Subunit Structure: Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3.
Post-translational Modifications: Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 58 kD
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
rab11 family-interacting protein 2 isoform 2
NCBI Official Synonym Full Names
RAB11 family interacting protein 2
NCBI Official Symbol
RAB11FIP2
NCBI Official Synonym Symbols
nRip11; Rab11-FIP2
NCBI Protein Information
rab11 family-interacting protein 2
UniProt Protein Name
Rab11 family-interacting protein 2
Protein Family
UniProt Gene Name
RAB11FIP2
UniProt Synonym Gene Names
KIAA0941; Rab11-FIP2
Uniprot Description
A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
Research Articles on RAB11FIP2
Similar Products
Product Notes
The RAB11FIP2 rab11fip2 (Catalog #AAA9605824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB11FIP2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAB11FIP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RAB11FIP2 rab11fip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB11FIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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