Mouse anti-Mouse FXYD Domain-containing Ion Transport Regulator 1 Monoclonal Antibody | anti-FXYD3 antibody

FXYD Domain-containing Ion Transport Regulator 1, phosphorylated (Ser68) (Phospholemman, FLM, Fxyd1, Plm)

Cat. Num. AAA631758

• Gene Names: FXYD3; MAT8; PLML
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: FXYD Domain-containing Ion Transport Regulator 1; Monoclonal Antibody; phosphorylated (Ser68) (Phospholemman; FLM; Fxyd1; Plm); Anti -FXYD Domain-containing Ion Transport Regulator 1; anti-FXYD3 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i44
• Specificity: Recognizes mouse PLM when phosphorylated at Ser68.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-FXYD3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser68 of mouse PLM (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS631758 in 293 cells transfected with a plasmid encoding PLM.)

Related Product Information for anti-FXYD3 antibody

PLM (FXYD1)is a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.

Product Categories/Family for anti-FXYD3 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 5174635
• NCBI GeneID: 5349
• NCBI Accession #: NP_005962.1
• NCBI GenBank Nucleotide #: NM_005971.3
• UniProt Accession #: Q14802; Q13211; Q6IB59; A6NDE0; C9JDU2; F5H174; F8WB34
• Molecular Weight: 9,263 Da
• NCBI Official Full Name: FXYD domain-containing ion transport regulator 3 isoform 1
• NCBI Official Synonym Full Names: FXYD domain containing ion transport regulator 3
• NCBI Official Symbol: FXYD3
• NCBI Official Synonym Symbols: MAT8; PLML
• NCBI Protein Information: FXYD domain-containing ion transport regulator 3; phospholemman-like protein; mammary tumor 8 kDa protein; chloride conductance inducer protein Mat-8
• UniProt Protein Name: FXYD domain-containing ion transport regulator 3
• Protein Family: FXYD domain-containing ion transport regulator
• UniProt Gene Name: FXYD3
• UniProt Synonym Gene Names: MAT8; PLML
• UniProt Entry Name: FXYD3_HUMAN

NCBI Description

This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Uniprot Description

FXYD3: Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May be a modulator capable of activating endogenous oocyte channels. Belongs to the FXYD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane

Molecular Function: chloride channel activity; ATPase binding

Biological Process: regulation of catalytic activity; chloride transport; transmembrane transport