Rabbit anti-Human Synuclein, alpha Polyclonal Antibody | anti-SNCA antibody

Synuclein, alpha, CT (Non-A beta Component Of AD Amyloid, Non-A4 Component Of Amyloid Precursor, NACP, SNCA, PARK1) (MaxLight 750)

Cat. Num. AAA6501886

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Synuclein; alpha; Polyclonal Antibody; CT (Non-A beta Component Of AD Amyloid; Non-A4 Component Of Amyloid Precursor; NACP; SNCA; PARK1) (MaxLight 750); anti-SNCA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human alpha Synuclein.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-SNCA antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide in the C-terminal region of human Alpha-synuclein that recognizes both NACP112 and NCAP140 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Park1 (arrow) using rabbit polyclonal Park1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Park1 gene.)

Western Blot (WB)

(Western blot analysis of anti-Alpha-synuclein Pab in mouse brain tissue lysate. Alpha-synuclein (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-SNCA antibody

Antibodies; Synuclein

References

1. Kumru, H., et al., Ann. Neurol. 56(4):599-603 (2004). 2. Pigullo, S., et al., Parkinsonism Relat. Disord. 10(6):357-362 (2004). 3. Yao, D., et al., Proc. Natl. Acad. Sci. USA 101(29):10810-10814 (2004). 4. West, A.B., et al., J. Biol. Chem. 279(28):28896-28902 (2004). 5. Wang, F., et al., Genes Chromosomes Cancer 40(2):85-96 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 6806898
• NCBI GeneID: 6622
• NCBI Accession #: NP_009292.1; NP_000336.1
• NCBI GenBank Nucleotide #: NM_007308.2
• NCBI Official Full Name: alpha-synuclein isoform NACP112
• NCBI Official Synonym Full Names: synuclein alpha
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein
• Protein Family: Alpha-synuclein

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]