Rabbit Syndecan 4 Polyclonal Antibody | anti-SDC4 antibody

Anti-Syndecan 4 Antibody

Cat. Num. AAA177756

• Gene Names: SDC4; SYND4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Syndecan 4; Polyclonal Antibody; Anti-Syndecan 4 Antibody; Syndecan-4; Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; SDC4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan; ryudocan); syndecan proteoglycan 4; Syndecan4; anti-SDC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 198

• Applicable Applications for anti-SDC4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; WB: The detection limit for Syndecan 4 i approximately 0.1ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin section in 10mM citrate, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.; Optimal dilutions should be be determined by end users.; ; Anitbody can be supported by Chemiluminescent Kit MBS176460, supported by MBS176451 in IHC-P.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan 4 (172-198aa MKKKDEGSYDLGKKPIYKKAPTNEFYA), identical to the related mouse and rat sequences.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Syndecan 4 Picoband antibody, MBS177756, Western blottingAll lanes: Anti Syndecan 4 (MBS177756) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: Rat Lung Tissue Lysate at 50ugPredicted bind size: 22KDObserved bind size: 22KD)

Immunohistochemistry (IHC)

(Anti- Syndecan 4 Picoband antibody, MBS177756, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- Syndecan 4 Picoband antibody, MBS177756, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- Syndecan 4 Picoband antibody, MBS177756, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-SDC4 antibody

Syndecan 4 is also known as SDC4. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is amember of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecanreceptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein andparticipates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures ofonly two have been described to date. These two represent the major variants of this gene and encode the same protein.

References

1. "Entrez Gene: SDC4 syndecan 4". 2. Woods A, Couchman JR (May 1998). "Syndecans: synergistic activators of cell adhesion". Trends Cell Biol. 8 (5): 189-92.

NCBI and Uniprot Product Information

• NCBI GI #: 38201675
• NCBI GeneID: 6385
• NCBI Accession #: NP_002990.2
• NCBI GenBank Nucleotide #: NM_002999.3
• UniProt Accession #: P31431; O00773; Q16833; Q53FN9; Q6FGN3
• NCBI Official Full Name: syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: SDC4
• NCBI Official Synonym Symbols: SYND4
• NCBI Protein Information: syndecan-4
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: SDC4
• UniProt Synonym Gene Names: SYND4
• UniProt Entry Name: SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: cell surface; costamere; focal adhesion; Golgi lumen; integral to plasma membrane; lipid raft; lysosomal lumen; plasma membrane

Molecular Function: fibronectin binding; protein binding; protein kinase C binding

Biological Process: cell migration; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inner ear receptor stereocilium organization and biogenesis; neural tube closure; positive regulation of focal adhesion formation; positive regulation of protein kinase activity; positive regulation of stress fiber formation; retinoid metabolic process; signal transduction; ureteric bud development; wound healing