Rabbit anti-Human Syndecan-1/CD138 Polyclonal Antibody | anti-SDC1 antibody

Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal

Cat. Num. AAA8118885

• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Syndecan-1/CD138; Polyclonal Antibody; Anti-Syndecan-1/CD138 Antibody; Rabbit Polyclonal; Syndecan-1/CD138/SDC1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CD138 Antibody; Anti-SDC Antibody; Anti-SYND1 Antibody; Anti-syndecan Antibody; Anti-Syndecan-1 Antibody; syndecan 1; anti-SDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Syndecan-1/CD138/SDC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-SDC1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human Syndecan-1/CD138/SDC1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Syndecan-1/CD138/SDC1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-CD138 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SDC1 antibody

Background: Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.

References

Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.