Western Blot (WB)
(Anti-SYT1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lane B: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:56 kDa)
Rabbit anti-Human Synaptotagmin 1/SYT1 Polyclonal Antibody | anti-SYT1 antibody
Anti-Synaptotagmin 1/SYT1 Antibody, Rabbit Polyclonal
Gene Names
SYT1; P65; SYT; SVP65
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Synaptotagmin 1/SYT1; Polyclonal Antibody; Anti-Synaptotagmin 1/SYT1 Antibody; Rabbit Polyclonal; Synaptotagmin 1/SYT1 Antibody; Rabbit PAb; Antigen Affinity Purified; synaptotagmin I; Anti-P65 Antibody; Anti-SVP65 Antibody; Anti-SYT Antibody; anti-SYT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Synaptotagmin 1/SYT1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SYT1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human Synaptotagmin 1/SYT1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Synaptotagmin 1/SYT1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-SYT1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lane B: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:56 kDa)
Western Blot (WB)
(Anti-SYT1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lane B: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:56 kDa)
Related Product Information for anti-SYT1 antibody
Synaptotagmin 1 (Syt1) is a synaptic vesicle integral membrane protein that regulates neurotransmitter release by activating fast synchronous fusion and suppressing slower asynchronous release. The cytoplasmic C2 domains of Syt1 interact with SNAREs and plasma membrane phospholipids in a Ca(2+)-dependent manner and can substitute for full-length Syt1 in vitro membrane fusion assays. Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum-plasma membrane (ER-PM) contact sites in leaf and root cells. The ER-PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cells. In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, and endocytosis. Arabidopsis SYT1 was reported to be essential for maintaining cell integrity and virus movement. The SYT1 gene is a genome and transcriptome-wide association hit in cognitive performance, personality, and cocaine use disorder (CUD) studies.
Product Categories/Family for anti-SYT1 antibody
References
Siao W, et al. (2016) Arabidopsis syt1 maintains stability of cortical endoplasmic reticulum networks and vap27-1-enriched endoplasmic reticulum-plasma membrane contact sites. J Exp Bot 67 (21): 6161-6171.Lee J, et al. (2015) Transmembrane tethering of synaptotagmin to synaptic vesicles controls multiple modes of neurotransmitter release. Proc Natl Acad Sci U S A 112 (12): 3793-3798.da Silva BS, et al. (2019) The association between syt1-rs2251214 and cocaine use disorder further supports its role in psychiatry. Prog Neuropsychopharmacol Biol Psychiatry 94 109642.Baker K, et al. (2018) Syt1-associated neurodevelopmental disorder: A case series. Brain 141 (9): 2576-2591.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
422
NCBI Official Full Name
synaptotagmin-1
NCBI Official Synonym Full Names
synaptotagmin I
NCBI Official Symbol
SYT1
NCBI Official Synonym Symbols
P65; SYT; SVP65
NCBI Protein Information
synaptotagmin-1; sytI; synaptotagmin 1
UniProt Protein Name
Synaptotagmin-1
Protein Family
UniProt Gene Name
SYT1
UniProt Synonym Gene Names
SVP65; SYT; SytI
UniProt Entry Name
SYT1_HUMAN
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Product Notes
The SYT1 syt1 (Catalog #AAA8103288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Synaptotagmin 1/SYT1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptotagmin 1/SYT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SYT1 syt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptotagmin 1/SYT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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