Goat IGFBP6 Polyclonal Antibody | anti-IGFBP6 antibody

Goat anti-IGFBP6 Antibody

Cat. Num. AAA420496

• Gene Names: IGFBP6; IBP6
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: IGFBP6; Polyclonal Antibody; Goat anti-IGFBP6 Antibody; insulin-like growth factor binding protein 6; IBP6; IGF binding protein 6; IGFBP6 antibody; insulin-like growth factor binding protein 6 antibody; IBP6 antibody; IGF binding protein 6 antibody; anti-IGFBP6 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RPQDVNRRDQQRNP
• Sequence Length: 240

• Applicable Applications for anti-IGFBP6 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx.25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx.29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.
• Immunogen: Peptide with sequence C-RPQDVNRRDQQRNP, from the internal region of the protein sequence according to NP_002169.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing IGFBP6 (RC204060) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

• NCBI GI #: 11321593
• NCBI GeneID: 3489
• NCBI Accession #: NP_002169.1
• NCBI GenBank Nucleotide #: NM_002178.2
• Molecular Weight: 25,322 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 6
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 6
• NCBI Official Symbol: IGFBP6
• NCBI Official Synonym Symbols: IBP6
• NCBI Protein Information: insulin-like growth factor-binding protein 6
• UniProt Protein Name: Insulin-like growth factor-binding protein 6
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP6
• UniProt Synonym Gene Names: IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
• UniProt Entry Name: IBP6_HUMAN

Uniprot Description

IGFBP6: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region; extracellular space; Golgi apparatus

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding; receptor binding

Biological Process: cellular protein metabolic process; negative regulation of cell proliferation; regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway; signal transduction