Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SWAP70 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit anti-Human, Mouse SWAP70 Polyclonal Antibody | anti-SWAP70 antibody
SWAP70 Polyclonal Antibody
Gene Names
SWAP70; HSPC321; SWAP-70
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
SWAP70; Polyclonal Antibody; SWAP70 Polyclonal Antibody; HSPC321; SWAP-70; anti-SWAP70 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
HKEARQRRKELRKKQLAEQEELERQMKELQAANESKQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTTEAEKQELENQRVLKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLIEPGSKNPHLITNWGPAAFTEAE
Sequence Length
527
Applicable Applications for anti-SWAP70 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human SWAP70
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cell membrane, Cell projection, Cytoplasm, Nucleus, lamellipodium
Positive Samples
293T, A549, LO2
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SWAP70 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SWAP70 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Product Categories/Family for anti-SWAP70 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 68kDa
Observed: 69kDa
Observed: 69kDa
NCBI Official Full Name
switch-associated protein 70 isoform 2
NCBI Official Synonym Full Names
switching B cell complex subunit SWAP70
NCBI Official Symbol
SWAP70
NCBI Official Synonym Symbols
HSPC321; SWAP-70
NCBI Protein Information
switch-associated protein 70
UniProt Protein Name
Switch-associated protein 70
Protein Family
UniProt Gene Name
SWAP70
UniProt Synonym Gene Names
KIAA0640; SWAP-70
Uniprot Description
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion ().
Research Articles on SWAP70
Similar Products
Product Notes
The SWAP70 swap70 (Catalog #AAA9134849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SWAP70 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SWAP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the SWAP70 swap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HKEARQRRKE LRKKQLAEQE ELERQMKELQ AANESKQQEL EAVRKKLEEA ASRAAEEEKK RLQTQVELQA RFSTELEREK LIRQQMEEQV AQKSSELEQY LQRVRELEDM YLKLQEALED ERQARQDEET VRKLQARLLE EESSKRAELE KWHLEQQQAI QTTEAEKQEL ENQRVLKEQA LQEAMEQLEQ LELERKQALE QYEEVKKKLE MATNKTKSWK DKVAHHEGLI RLIEPGSKNP HLITNWGPAA FTEAE. It is sometimes possible for the material contained within the vial of "SWAP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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