Goat SURVIVIN Polyclonal Antibody | anti-BIRC5 antibody

GOAT ANTI HUMAN SURVIVIN (C-TERMINAL)

Cat. Num. AAA224113

• Gene Names: BIRC5; API4; EPR-1
• Applications: ELISA, Western Blot
• Synonyms: SURVIVIN; Polyclonal Antibody; GOAT ANTI HUMAN SURVIVIN (C-TERMINAL); anti-BIRC5 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-terminal (CT) region of human Survivin, otherwise known as TIAP/BIRC5, a novel inhibitor of apoptosis (IAP) protein, and component of the chromosomal passenger complex, which plays a key role in the regulation of mitosis. ; ; Survivin is only expressed during the G2-M cell cycle phase, and is predominantly expressed in foetal tissues, and in many human cancers, including adenocarcinomas and high-grade lymphomas. The lack of survivin expression by normal non-malignant cells, makes survivin an important therapeutic target for cancer.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 137

• Applicable Applications for anti-BIRC5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 18kDa in MOLT4 cell lysates.; ELISA: Minimum Dilution: 1/16000; Western Blotting: Minimum Dilution: 0.01; Maximum Dilution: 0.5ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide sequence CVRRAIEQLAAMD from the C-Terminal region of Survivin (NP_001159.2; NP_001012271.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human Survivin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(MOLT4 cell lysate probed with Goat anti human Survivin C-Terminal (MBS224113))

Related Product Information for anti-BIRC5 antibody

MBS224113 specifically recognises an epitope within the C-terminal (CT) region of human Survivin, otherwise known as TIAP/BIRC5, a novel inhibitor of apoptosis (IAP) protein, and component of the chromosomal passenger complex, which plays a key role in the regulation of mitosis. Survivin is only expressed during the G2-M cell cycle phase, and is predominantly expressed in foetal tissues, and in many human cancers, including adenocarcinomas and high-grade lymphomas. The lack of survivin expression by normal non-malignant cells, makes survivin an important therapeutic target for cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 59859880
• NCBI GeneID: 332
• NCBI Accession #: NP_001012270.1
• NCBI GenBank Nucleotide #: NM_001012270.1
• UniProt Accession #: O15392; Q2I3N8; Q4VGX0; Q53F61; Q5MGC6; Q6FHL2; Q75SP2; Q9P2W8; A2SUH6; B2R4R1
• Molecular Weight: 8,490 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 2
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 5
• NCBI Official Symbol: BIRC5
• NCBI Official Synonym Symbols: API4; EPR-1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5; apoptosis inhibitor 4; apoptosis inhibitor survivin; survivin variant 3 alpha
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC5
• UniProt Synonym Gene Names: API4; IAP4
• UniProt Entry Name: BIRC5_HUMAN

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; spindle microtubule; cytoplasmic microtubule; cytoplasm; spindle; midbody; nucleus; cytosol; interphase microtubule organizing center; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein binding; enzyme binding; protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; chaperone binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; cofactor binding; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; regulation of signal transduction; protein ubiquitination; cytokinesis; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; positive regulation of exit from mitosis; negative regulation of apoptosis