Rabbit anti-Human Survivin Polyclonal Antibody | anti-BIRC5 antibody

Survivin Antibody

Cat. Num. AAA9402625

• Gene Names: BIRC5; API4; EPR-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Survivin; Polyclonal Antibody; Survivin Antibody; TIAP; anti-BIRC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 142

• Applicable Applications for anti-BIRC5 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids near the carboxy terminus of human survivin.
• Target Name: Survivin
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of MOLT4 cells using survivin at 10 ug/mL.)

Related Product Information for anti-BIRC5 antibody

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.

Product Categories/Family for anti-BIRC5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 59859878
• NCBI GeneID: 332
• NCBI Accession #: NP_001159
• NCBI GenBank Nucleotide #: NM_001168.2
• Molecular Weight: 8,490 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 1
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 5
• NCBI Official Symbol: BIRC5
• NCBI Official Synonym Symbols: API4; EPR-1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC5
• UniProt Synonym Gene Names: API4; IAP4
• UniProt Entry Name: BIRC5_HUMAN

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; cytoplasmic microtubule; spindle microtubule; cytoplasm; spindle; midbody; interphase microtubule organizing center; nucleus; cytosol; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; metal ion binding; microtubule binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cysteine protease inhibitor activity; cofactor binding; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; spindle checkpoint; transcription, DNA-dependent; establishment of chromosome localization; protein ubiquitination; regulation of signal transduction; cytokinesis; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of exit from mitosis