Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using PRKAG1 antibody.)
Rabbit AMPKgamma1 Polyclonal Antibody | anti-AMPKgamma1 antibody
AMPKgamma1 Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using PRKAG1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using PRKAG1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PRKAG1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PRKAG1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.
Protein type: Protein kinase, regulatory subunit; Autophagy
Chromosomal Location of Human Ortholog: 12q12-q14
Cellular Component: membrane; nucleus; cytosol; AMP-activated protein kinase complex
Molecular Function: AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; ADP binding; protein kinase binding; ATP binding; protein kinase activity; AMP binding
Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; positive regulation of protein kinase activity; regulation of glycolysis; insulin receptor signaling pathway; spermatogenesis; cell cycle arrest; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process
Research Articles on AMPKgamma1
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Product Notes
The AMPKgamma1 prkag1 (Catalog #AAA3012041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMPKgamma1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AMPKgamma1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the AMPKgamma1 prkag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPKgamma1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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