Rabbit anti-Mouse Superoxide Dismutase 3/EC-SOD/Sod3 Polyclonal Antibody | anti-Sod3 antibody
Anti-Superoxide Dismutase 3/EC-SOD/Sod3 Antibody
Gene Names
Sod3; EC-SOD; AI314465
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
Superoxide Dismutase 3/EC-SOD/Sod3; Polyclonal Antibody; Anti-Superoxide Dismutase 3/EC-SOD/Sod3 Antibody; Sod3; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1. 15. 1. 1; superoxide dismutase 3; extracellular; anti-Sod3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Superoxide Dismutase 3/EC-SOD/Sod3 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-Sod3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Mouse|
IHC-P: 2-5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
IHC-P: 2-5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
Immunogen
E Coli-derived mouse Sod3 recombinant protein (Position: V36-L101).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-Sod3 antibody
SOD3 (SUPEROXIDE DISMUTASE 3), also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15. 3-p15. 1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
References
1. Folz, R. J., Crapo, J. D. Extracellular superoxide dismutase (SOD3): tissue-specific expression, genomic characterization, and computer-assisted sequence analysis of the human EC SOD gene. Genomics 22: 162-171, 1994.
2. Hjalmarsson, K., Marklund, S. L., Engstrom, A., Edlund, T. Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase. Proc. Nat. Acad. Sci. 84: 6340-6344, 1987.
3. Stern, L. F., Chapman, N. H., Wijsman, E. M., Altherr, M. R., Rosen, D. R. Assignment of SOD3 to human chromosome band 4p15. 3-p15. 1 with somatic cell and radiation hybrid mapping, linkage mapping, and fluorescent in-situ hybridization. Cytogenet. Genome Res. 101: 178 only, 2003.
2. Hjalmarsson, K., Marklund, S. L., Engstrom, A., Edlund, T. Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase. Proc. Nat. Acad. Sci. 84: 6340-6344, 1987.
3. Stern, L. F., Chapman, N. H., Wijsman, E. M., Altherr, M. R., Rosen, D. R. Assignment of SOD3 to human chromosome band 4p15. 3-p15. 1 with somatic cell and radiation hybrid mapping, linkage mapping, and fluorescent in-situ hybridization. Cytogenet. Genome Res. 101: 178 only, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,392 Da
NCBI Official Full Name
extracellular superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 3, extracellular
NCBI Official Symbol
Sod3
NCBI Official Synonym Symbols
EC-SOD; AI314465
NCBI Protein Information
extracellular superoxide dismutase [Cu-Zn]
UniProt Protein Name
Extracellular superoxide dismutase [Cu-Zn]
Protein Family
UniProt Gene Name
Sod3
UniProt Synonym Gene Names
EC-SOD
UniProt Entry Name
SODE_MOUSE
Uniprot Description
SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.
Protein type: Oxidoreductase; Secreted; EC 1.15.1.1; Secreted, signal peptide
Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; nucleus; trans-Golgi network
Molecular Function: antioxidant activity; copper ion binding; metal ion binding; oxidoreductase activity; protein binding; superoxide dismutase activity; zinc ion binding
Biological Process: response to copper ion; response to hypoxia; superoxide metabolic process
Research Articles on Sod3
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Product Notes
The Sod3 sod3 (Catalog #AAA1753463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Superoxide Dismutase 3/EC-SOD/Sod3 Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 3/EC-SOD/Sod3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse| IHC-P: 2-5ug/ml|Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse| Direct ELISA: 0.1-0.5ug/ml|Mouse|. Researchers should empirically determine the suitability of the Sod3 sod3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 3/EC-SOD/Sod3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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