Rabbit anti-Human Superoxide Dismutase 3 Polyclonal Antibody | anti-SOD3 antibody

Anti-Superoxide Dismutase 3 antibody

Cat. Num. AAA176196

• Gene Names: SOD3; EC-SOD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Superoxide Dismutase 3; Polyclonal Antibody; Anti-Superoxide Dismutase 3 antibody; Extracellular superoxide dismutase[Cu-Zn]; superoxide dismutase 3; extracellular; EC SOD antibody; EC-SOD antibody; Extracellular superoxide dismutase[Cu Zn] antibody; Extracellular superoxide dismutase[Cu-Zn] antibody; Extracellular superoxide dismutase antibody; Extracellular superoxide dismutase precursor antibody; MGC20077 antibody; SOD 3 antibody; SOD3 antibody; SODE_HUMAN antibody; Superoxide dismutase 3 extracellular antibody; anti-SOD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 240

• Applicable Applications for anti-SOD3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Application Notes: Western blot; Concentration: 0.1-0.5 ug/mL; Tested Species: Hu; ; Immunohistochemistry (Paraffin-embedded Section); Concentration: 0.5- 1 ug/mL; Tested Species: Hu; Antigen Retrieval: By Heat; ; Immunohistochemistry(Frozen Section):; Concentration: 0.5- 1 ug/mL; Tested Species: Hu; ; Immunocytochemistry:; Concentration: 0.5- 1ug/mL; Tested Species: Human; ; Tested Species: In-house tested species with positive results; ; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SOD3 (41-56aa KVTEIWQEVMQRRDDD)
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-SOD3 antibody, MBS176196, Western blottingLane 1: Human Placenta Tissue LysateLane 2: A549 Cell LysateLane 3: MM231 Cell LysateLane 4: MCF-7 Cell Lysate )

Related Product Information for anti-SOD3 antibody

Description: Rabbit IgG polyclonal antibody for Extracellular superoxide dismutase[Cu-Zn](SOD3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Background: SOD3(SUPEROXIDE DISMUTASE 3) also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase(SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al.(1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al.(2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix(ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.

NCBI and Uniprot Product Information

• NCBI GI #: 108885292
• NCBI GeneID: 6649
• UniProt Accession #: P08294; Q5U781; Q6FHA2
• NCBI Official Full Name: Extracellular superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 3, extracellular
• NCBI Official Symbol: SOD3
• NCBI Official Synonym Symbols: EC-SOD
• NCBI Protein Information: extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Extracellular superoxide dismutase [Cu-Zn]
• Protein Family: Extracellular superoxide dismutase
• UniProt Gene Name: SOD3
• UniProt Synonym Gene Names: EC-SOD
• UniProt Entry Name: SODE_HUMAN

NCBI Description

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Secreted; Secreted, signal peptide; EC 1.15.1.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia