Rabbit anti-Human, Mouse SUB1 Polyclonal Antibody | anti-SUB1 antibody

SUB1 Polyclonal Antibody

Cat. Num. AAA3011930

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SUB1; Polyclonal Antibody; SUB1 Polyclonal Antibody; Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14; RPO2TC1; anti-SUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SUB1 polyclonal antibody detects endogenous levels of SUB1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 127

• Applicable Applications for anti-SUB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:100; IP: 1:20-1:50
• Immunogen: Recombinant full length Human SUB1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using SUB1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using SUB1 antibody.)

Related Product Information for anti-SUB1 antibody

RNA polymerase II (RNAPII) holoenzyme contains numerous proteins that largely consist of RNA processing factors, RNA helicase, general transcription factors and SRB co-activating complexes. RNAPII mediated basal- and gene-specific transcriptional activation requires the association of various cofactors that includes PC4 (human Positive cofactor 4). Positive cofactor 4 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. Positive cofactor 4 induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Addition-ally, Positive cofactor 4 is phosphorylated by TFIID and TFIIH, which releases Positive cofactor 4 from the DNA promoter region and thereby inhibits the assembly of Positive cofactor 4 into the transcriptional promoting complex and blocks transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 1709514
• UniProt Accession #: P53999
• Molecular Weight: ~ 14kDa
• NCBI Official Full Name: Activated RNA polymerase II transcriptional coactivator p15
• UniProt Protein Name: Activated RNA polymerase II transcriptional coactivator p15
• Protein Family: Subtilisin
• UniProt Gene Name: SUB1
• UniProt Synonym Gene Names: PC4; RPO2TC1; PC4
• UniProt Entry Name: TCP4_HUMAN

Uniprot Description

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: DNA-binding; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function: protein binding; transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter