Goat STS2/TULA Polyclonal Antibody | anti-UBASH3A antibody

Goat anti-STS2/TULA (internal) Antibody

Cat. Num. AAA420681

• Gene Names: UBASH3A; TULA; CLIP4; STS-2; TULA-1
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: STS2/TULA; Polyclonal Antibody; Goat anti-STS2/TULA (internal) Antibody; Goat Anti-STS2 / TULA (N Terminus) Antibody; TULA; STS2 CBL-interacting protein 4; UBASH3A; ubiquitin associated and SH3 domain containing; A; T-cell ubiquitin ligand protein; Suppressor of T-cell receptor signaling 2; CLIP4; TULA antibody; STS2 CBL-interacting protein 4 antibody; UBASH3A antibody; A antibody; T-cell ubiquitin ligand protein antibody; Suppressor of T-cell receptor signaling 2 antibody; CLIP4 antibody; STS2 / TULA (internal); anti-UBASH3A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QQCSTPDGKYYRPD
• Sequence Length: 661

• Applicable Applications for anti-UBASH3A antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration, 2.5ug/ml.; Western Blot: Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence QQCSTPDGKYYRPD, from the internal region of the protein sequence according to NP_061834.1; NP_001001895.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 9507221
• NCBI GeneID: 53347
• NCBI Accession #: NP_061834.1; NP_001001895.1
• NCBI GenBank Nucleotide #: NM_018961.3
• Molecular Weight: 59,167 Da
• NCBI Official Full Name: ubiquitin-associated and SH3 domain-containing protein A isoform 1
• NCBI Official Synonym Full Names: ubiquitin associated and SH3 domain containing A
• NCBI Official Symbol: UBASH3A
• NCBI Official Synonym Symbols: TULA; CLIP4; STS-2; TULA-1
• NCBI Protein Information: ubiquitin-associated and SH3 domain-containing protein A
• UniProt Protein Name: Ubiquitin-associated and SH3 domain-containing protein A
• Protein Family: Ubiquitin-associated and SH3 domain-containing protein
• UniProt Gene Name: UBASH3A
• UniProt Synonym Gene Names: STS2; CLIP4; STS-2; TULA-1
• UniProt Entry Name: UBS3A_HUMAN

NCBI Description

This gene encodes one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. Both family members can negatively regulate T-cell signaling. This family member can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

STS2: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytosol; nucleus

Molecular Function: protein binding

Biological Process: negative regulation of T cell receptor signaling pathway; regulation of cytokine production