Mouse CD161b/c Monoclonal Antibody | anti-NK-1.1 antibody

MOUSE ANTI MOUSE NK-1.1 ANTIGEN

Cat. Num. AAA212065

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD161b/c; Monoclonal Antibody; MOUSE ANTI MOUSE NK-1.1 ANTIGEN; anti-NK-1.1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: PK136
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 223

• Applicable Applications for anti-NK-1.1 antibody: Cytotoxic Assays (CT), Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. ; Cytotoxic Assays: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Flow Cytometry: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Spleen and bone marrow cells from CE mice; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of New Zealand Black mouse peripheral blood lymphocytes with Mouse anti Mouse NK Cells:RPE (MBS213569))

Related Product Information for anti-NK-1.1 antibody

Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 (clone PK136) recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.

NCBI and Uniprot Product Information

• NCBI GI #: 128356
• Molecular Weight: 25,158 Da
• NCBI Official Full Name: Killer cell lectin-like receptor subfamily B member 1B allele A
• UniProt Protein Name: Killer cell lectin-like receptor subfamily B member 1B allele A
• UniProt Gene Name: Klrb1b
• UniProt Synonym Gene Names: Ly55b; Nkrp1b; Ly-55b; NKR-P1B
• UniProt Entry Name: KRBBA_MOUSE

Uniprot Description

Receptor for CLEC2D/OCIL. Ligand-binding contributes to inhibition of cytotoxic natural killer (NK) cells. May mediate MHC class I-independent "missing-self" recognition of allografts, tumor cells and virus-infected cells.