Rabbit anti-Human RBPJL Polyclonal Antibody | anti-Rbpjl antibody

RBPJL, NT (RBPJL, RBPL, RBPSUHL, Recombining binding protein suppressor of hairless-like protein, Transcription factor RBP-L)

Cat. Num. AAA6013339

• Gene Names: Rbpjl; RBP-L; Rbpsuhl
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RBPJL; Polyclonal Antibody; NT (RBPJL; RBPL; RBPSUHL; Recombining binding protein suppressor of hairless-like protein; Transcription factor RBP-L); Anti -RBPJL; anti-Rbpjl antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Rbpjl antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: RBPJL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RBPJL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) using MBS6013339 .This demonstrates that MBS6013339 detected the RBPJL protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6013339 followed by peroxidase conjugation of the secondary antibody and DAB staining.T his data demonstrates the use of MBS6013339 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram) using MBS6013339 compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Rbpjl antibody

In mouse, recombining binding protein L (RBP-L) is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signalling pathway transcription factor RBP-J. However, unlike RBP-J, RBP-L does not interact with Notch receptors. RBP-L has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2 (EBNA2). The protein encoded by this gene is similar in sequence to the mouse RPB-L protein and Drosophila suppressor of hairless protein.

Product Categories/Family for anti-Rbpjl antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 6677689
• NCBI GeneID: 19668
• NCBI Accession #: NP_033062.1
• NCBI GenBank Nucleotide #: NM_009036.1
• UniProt Accession #: O08674
• Molecular Weight: 56,780 Da
• NCBI Official Full Name: recombining binding protein suppressor of hairless-like protein
• NCBI Official Synonym Full Names: recombination signal binding protein for immunoglobulin kappa J region-like
• NCBI Official Symbol: Rbpjl
• NCBI Official Synonym Symbols: RBP-L; Rbpsuhl
• NCBI Protein Information: recombining binding protein suppressor of hairless-like protein; RBP-J kappa-like; transcription factor RBP-L
• UniProt Protein Name: Recombining binding protein suppressor of hairless-like protein
• Protein Family: Recombining binding protein suppressor of hairless-like protein
• UniProt Gene Name: Rbpjl
• UniProt Synonym Gene Names: Rbpl; Rbpsuhl
• UniProt Entry Name: RBPJL_MOUSE

Uniprot Description

RBPJL: Putative transcription factor, which cooperates with EBNA2 to activate transcription. Belongs to the Su(H) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; nucleus

Molecular Function: DNA binding; chromatin binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent