Rabbit anti-Human Stromal Cell Derived Factor 1alpha Polyclonal Antibody | anti-CXCL12 antibody

Stromal Cell Derived Factor 1alpha (SDF1 alpha, SDF1a, SDF-1a, Chemokine (C-X-C Motif) Ligand 12, CXCL12, hIRH, Pre-B cell Growth-stimulating Factor, PBSF, SCYB12, TLSF-a, TPAR1)

Cat. Num. AAA614277

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Stromal Cell Derived Factor 1alpha; Polyclonal Antibody; Stromal Cell Derived Factor 1alpha (SDF1 alpha; SDF1a; SDF-1a; Chemokine (C-X-C Motif) Ligand 12; CXCL12; hIRH; Pre-B cell Growth-stimulating Factor; PBSF; SCYB12; TLSF-a; TPAR1); Anti -Stromal Cell Derived Factor 1alpha (SDF1 alpha; anti-CXCL12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Human SDF-1alpha
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.5X PBS, pH 7.4. Reconstitute with sterile, distilled, deionized water or sterile 40-50% glycerolto a concentration of 0.2mg/ml

• Applicable Applications for anti-CXCL12 antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in ELISA, Neutralization and Western Blot.; Dilution: ELISA: >0.5ug/ml Detects hSDF-1-alpha by direct ELISA (using 100µl/well antibody solution) Allows the detection of 0.2-0.4 ng/well of recombinant hSDF-1-alpha.; Neutralization: 2-4ug/ml Will yield one-half maximal inhibition [; NK50]of the biological activity of hSDF1-1a (100ng/ml).; Western Blot: 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.; Optimal dilution determined by the researcher.
• Immunogen: Highly purified, recombinant SDF-1alpha
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CXCL12 antibody

Chemokine SDF-1 is highly conserved among species and belongs to a large family of small, structurally related cytokines. It is a chemoattractant for T lymphocytes, monocytes, and hemopoietic stem cells and is constitutively expressed in bone marrow-derived stromal cells. Recent reports indicate that SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. SDF-1a and SDF-1b, are generated by alternative RNA splicing of transcripts encoded by a single gene. SDF-1a differs from SDF-1b by four C-terminal amino acid residues absent in SDF-1a. As yet, no functional differences have been reported. Stromal Cell-Derived Factor-1 (SDF1) is a recently discovered protein belonging to the -chemokine (C-X-C) family of cytokines. Human SDF1 is an 8kD protein containing 68 amino acid residues.

Product Categories/Family for anti-CXCL12 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 40316924
• NCBI GeneID: 6387
• NCBI Accession #: NP_954637.1
• NCBI GenBank Nucleotide #: NM_199168.3
• UniProt Accession #: Q6ICW0; P48061; Q2L985; Q2L986; Q2L988; Q8NF00
• Molecular Weight: 10,103 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform alpha
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1; OTTHUMP00000019491; OTTHUMP00000019492; OTTHUMP00000216979; OTTHUMP00000216980; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
• UniProt Protein Name: CXCL12 protein
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: CXCL12
• UniProt Entry Name: Q6ICW0_HUMAN

NCBI Description

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.1

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding

Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; signal transduction; chemotaxis; germ cell development; induction of positive chemotaxis; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to mechanical stimulus; response to heat; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; immune response; positive regulation of endothelial cell proliferation; telencephalon cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To