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Western Blot (WB) (Western blot analysis of STK40 (arrow) using rabbit polyclonal STK40 (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK40 gene (Lane 2).)

Rabbit anti-Human STK40 Polyclonal Antibody | anti-STK40 antibody

STK40 Antibody (C-term)

Gene Names
STK40; SHIK; SgK495
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STK40; Polyclonal Antibody; STK40 Antibody (C-term); Serine/threonine-protein kinase 40; SINK-homologous serine/threonine-protein kinase; Sugen kinase 495; SgK495; SGK495; SHIK; anti-STK40 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STK40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-403 amino acids from the C-terminal region of human STK40.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
372-403
Sequence Length
435
Applicable Applications for anti-STK40 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of STK40 (arrow) using rabbit polyclonal STK40 (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK40 gene (Lane 2).)

Western Blot (WB) (Western blot analysis of STK40 (arrow) using rabbit polyclonal STK40 (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK40 gene (Lane 2).)

Immunohistochemistry (IHC)

(STK40 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STK40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (STK40 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STK40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(STK40 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (STK40 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-STK40 antibody
STK40 may be a negative regulator of NF-kappa-B and p53-mediated gene transcription.
Product Categories/Family for anti-STK40 antibody
References
Greenman C., et.al., Nature 446:153-158(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49001
NCBI Official Full Name
serine/threonine-protein kinase 40 isoform 2
NCBI Official Synonym Full Names
serine/threonine kinase 40
NCBI Official Symbol
STK40
NCBI Official Synonym Symbols
SHIK; SgK495
NCBI Protein Information
serine/threonine-protein kinase 40
UniProt Protein Name
Serine/threonine-protein kinase 40
UniProt Gene Name
STK40
UniProt Synonym Gene Names
SGK495; SHIK; SgK495
UniProt Entry Name
STK40_HUMAN

Uniprot Description

SHIK: an ubiquitously expressed protein kinase of the CAMK family. Cytoplasmic. May be a negative regulator of NF-kappaB- and p53-mediated gene transcription.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; CAMK group; CAMK-Unique family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: glycogen metabolic process; respiratory system process; regulation of gene expression; regulation of MAPKKK cascade; multicellular organism growth; protein amino acid phosphorylation; alveolus development; negative regulation of apoptosis

Research Articles on STK40

Similar Products

Product Notes

The STK40 stk40 (Catalog #AAA9200955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 372-403. The STK40 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the STK40 stk40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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